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User Manual fluidlab R-300

 

 

 

Edition 202104 

 

 

 

 

 

 

 

67 

Vers.: 1.2 

9.2

 

Accessories 

 

Name 

Part number 

Description 

USB power supply unit 

10048 

external, type 31507W  
(inLine ®) 

USB connection cable 
[type A to type C] 

10049 

Length: 1.8 m 

acella20

 sample carrier 

10114 

Microscope sample carrier with a 
chamber height of 20 µm 

acella50

 sample carrier 

10056 

Microscope sample carrier with a 
chamber height of 50 µm 

acella100

 sample carrier 

10085 

Microscope sample carrier with a 
chamber height of 100 µm 

Cleaning swabs 

10078 

Plastic sticks with cellulose scraper 
(set of 12) 

Sample carrier adapter 01 

–

 

acella

 

10039 

Plastic insert for measuring 
chamber for acellas 

Sample carrier adapter 02 

–

 

cuvette

  

10135 

Plastic insert for measuring 
chamber for scattered light 
measurements with cuvettes  

Sample carrier adapter 03 

–

 

cuvette

 

10131 

Plastic insert for measuring 
chamber for absorption 
measurements with cuvettes 

Device case 

10054 

Lockable hard plastic case with 
internal padding, especially for the 

fluidlab 

R-300

 

with accessories 

 
 

 

Summary of Contents for fluidlab R-300

Page 1: ...User manual fluidlab R 300 ...

Page 2: ...for damages Section 97 of the Copyright Act Registered Trademarks All hardware and software names specified in this manual are registered trademarks or should be regarded as such The proper names of anvajo products and software are shown in bold Errors Great care has been taken in compiling the text and figures in this manual Nonetheless the possibility of errors cannot be fully excluded The compa...

Page 3: ...User Manual fluidlab R 300 4 Edition 202104 Vers 1 2 ...

Page 4: ...ergy saving Mode 19 5 Setting up the System 21 5 1 Settings Menu 21 5 2 Configuration of the WLAN Adapter 22 5 2 1 Description of Operating Modes 22 5 2 2 WLAN Mode 22 5 2 3 Hotspot Mode 23 5 2 4 Offline Mode 24 5 3 Software Update 24 5 4 Maintenance 25 5 5 Language Settings 25 5 6 Displaying system information 26 6 Carrying Out Measurements 27 6 1 Preliminary Remarks 27 6 2 Spectrometric Measurem...

Page 5: ...55 7 1 2 Connection via Hotspot 55 7 2 Data Export 56 7 3 Updates via datalab 56 8 Operating and Maintenance Instructions 57 8 1 Prohibitions on Use 57 8 2 Description of Use 57 8 2 1 User Groups 57 8 2 2 User Expertise 57 8 2 3 Service Life 57 8 3 Usage Location 58 8 4 Warranty Provisions 58 8 5 Cleaning 59 8 5 1 General 59 8 5 2 External Cleaning 60 8 5 3 Cleaning the Sensor and Measuring Chambe...

Page 6: ...roduct safely Follow the instructions for use Temperature restrictions permissible temperature range temperature limits for storage and transport Serial number Manufacturer USB port Standby switch CE mark Waste symbol the black bar indicates a key date regulation cf 2012 19 EU Direct current Biological Risks Nameplate with device specific information such as serial number manufacturer and system n...

Page 7: ...ify anvajo GmbH if any malfunctions faults or damage to the system are observed during operation Warning To avoid the risk of electric shock the system must be operated in its original condition closed on all sides The system must be operated within the nominal operating range specified in Chapter 9 1 Warning Use only original parts or parts recommended by the manufacturer to avoid damage to the s...

Page 8: ...ufacturer may lead to increased electromagnetic interference or reduced electromagnetic immunity of the system and could result in incorrect functioning Portable RF communication devices mobile phones radios should not be used at a distance of less than 30 cm from the system Failure to observe this instruction may lead to a reduction in performance The system has been tested in accordance with EN ...

Page 9: ...arged to at least 40 once every six months The battery should only be replaced by the manufacturer Chapter 8 7 Warning Return the battery in a discharged state see Chapter 8 9 Warning Many chargers consume power while they are plugged in at the socket even if they are not charging Disconnect the charger from the mains after charging 2 5 Radio Communications The system has been tested in accordance...

Page 10: ...ystem is in operation as this may damage the retina Warning The device shall only be used with the accessories specified by anvajo GmbH As far as possible the slider should be closed during the measuring process The device must be switched off while the measuring chamber shaft is being cleaned The biological effects arising from UV LEDs depend on the emitted wavelength the exposure time and the in...

Page 11: ...User Manual fluidlab R 300 12 Edition 202104 Vers 1 2 ...

Page 12: ...ance parameters Spectrometer for measuring extinction absorption and scattered light for creating calibration curves and kinetics plots Microscope for cell counts and viability measurements Touch screen for performance data see Chapter 9 1 Mains or battery operation possible for performance data see Chapter 9 1 WLAN or USB connection WLAN as client or hotspot USB for charging Display of measured v...

Page 13: ...ew FOV 2 3 mm x 2 3 mm and guarantees high statistical reliability for every test result The hologram is then processed by anvajo s intelligent mathematical reconstruction software which delivers high resolution images Robust and fast image signal processing and data parallel operations combined with a high level of computing power make efficient analysis of microscopic images possible directly in...

Page 14: ...dapter starter set Sample carrier adapter 02 Cuvette for scattered light measurements 6b Adapter starter set Sample carrier adapter 03 Cuvette for absorption measurements 7 Adapter key Tool for changing the adapter Note Please check that the product set is complete based on the delivery note Only products and accessories that are completely free of defects should be put into operation Damage must ...

Page 15: ...dlab R 300 on or off 3 Touch screen Interface for interaction with the device 4 Slider Protects the measuring chamber shown open in the illustration 5 Sample carrier adapter Sample carrier specific adapter exchangeable 6 Slot for sample carrier For inserting the sample carrier opening corresponds to the space between the adapter and the measuring chamber 7 Measuring chamber Holder for sample carri...

Page 16: ... display 2 Connect the device to the supplied USB charger the USB cable and a mains socket the same procedure as when charging the battery see Chapter 4 1 3 The power on off button see Figure 2 2 lights up briefly The device is no longer in shipping mode and can be switched on see Chapter 4 2 4 1 Charging the Built in Battery Warning Throughout the charging period the fluidlab R 300 must be placed...

Page 17: ... to the device with the USB cable see Chapter 4 1 1 Switch the unit on by pressing and holding down the power on off button Figure 2 2 for approx one second and then releasing it The device is switched on as soon as the power on off button lights up 2 Wait until the system has started This process takes approx 40 seconds Start up is complete when the main menu appears on the display The device is ...

Page 18: ... predefined period of inactivity first reduces the brightness of the display and then switches off the display backlight energy saving mode Apart from the display of the graphical user interface the device is fully functional in this mode Energy saving mode Automatically activated after approx 2 minutes of non use Display is dimmed after 2 minutes and then switched off Power on off button Figure 2...

Page 19: ...User Manual fluidlab R 300 20 Edition 202104 Vers 1 2 ...

Page 20: ...he Settings menu No Name Function 1 WLAN button Display WLAN settings see Chapter 5 2 2 WLAN switch Enable disable WLAN see Chapter 5 2 3 Hotspot button Display hotspot menu see Chapter 5 2 4 Hotspot switch Activate deactivate hotspot see Chapter 5 2 5 Update button Display Update menu see Chapter 5 3 6 Update indicator Shows available software updates see Chapter 5 3 7 Maintenance Sensor status a...

Page 21: ...Offline mode In this mode the WLAN adapter of your fluidlab R 300 is switched off WLAN and hotspot are disabled As a result wireless communication with other PCs or update servers is not possible see Chapter 5 2 4 Note Obstacles such as walls glass windows machines and liquid containers that are located between the fluidlab R 300 and the connected WLAN access point e g router or a network device c...

Page 22: ...e LIS connection In this case please contact your network administrator in advance 5 2 3 Hotspot Mode The following section describes how you can allow your fluidlab R 300 to create its own WLAN hotspot You can then add other WLAN enabled devices to this WLAN so that they can communicate with your fluidlab R 300 1 Open the settings menu of the fluidlab R 300 by tapping the menu icon 2 Tap the swit...

Page 23: ...n software bugs are removed Performance improvements are implemented Operating concepts are optimised The fluidlab R 300 checks automatically whether new software updates are available if there is an existing Internet connection see Chapter 5 2 2 Moreover the user can also manually initiate the update check by clicking on Check for updates The installation of these updates is explained below in th...

Page 24: ...as automatically restarted Note If the fluidlab R 300 is connected to a WLAN with Internet access the system will regularly and automatically check for any software updates The fluidlab R 300 notifies you that an update is available with a green dot on the menu icon and the update icon Note If a new software update is available a message window will appear within the update menu at the bottom righ...

Page 25: ...be applied and the display will change straightaway 5 6 Displaying system information You can display the system information for your device as follows 1 Open the settings menu of the fluidlab R 300 by tapping the menu icon 2 Tap on the menu item System 3 The system information will now be displayed The system information includes the following details The model name Device The serial number of th...

Page 26: ...n of 2 nm Full optical absorption measurements can be carried out effortlessly with sample carrier adapter 03 and scattered light measurements with sample carrier adapter 02 6 2 2 Using the Cuvettes Note For spectrometric measurements in the fluidlab R 300 you can use all quartz glass or plastic cuvettes with an outside area of 12 5 x 12 5 mm 10 x 10 mm inside that are suitable for the UV VIS meas...

Page 27: ...start of each series of measurements In general this blank contains the matrix of the sample without corresponding analytes 6 2 3 2 Determining the Extinction of Liquids 1 Prepare your samples and the respective blank liquids in the cuvettes and make them ready for the upcoming measurements 2 Open the slider on the fluidlab R 300 as described in Chapter 6 2 2 3 Insert sample carrier adapter 02 or ...

Page 28: ...e correct orientation Figure 4 Now start the measurement of your sample by tapping on the Continue button with a green background 11 The device now displays the result which was measured at the pre set wavelength 12 If you scroll down further the spectral graph of the liquid between 375 and 700 nm is also displayed Figure 6 Results screen of the Extinction application Note Spectral graph 375 700 n...

Page 29: ...ering a new value on the keyboard or by tapping on the plus or minus key Figure 7 Extinction spectrum of a liquid between 375 and 700 nm Note Real time measurement live mode You can use the live mode function to check whether the extinction value remains stable Figure 6 To do so tap on the button and slide it to the right Real time measurement is now activated green dot and the spectrometer will m...

Page 30: ...ll automatically be numbered consecutively and stored in the History If the option Re measure blank is chosen the device automatically starts to measure a blank again Figure 8 Optional blanking Note It is recommended to blank between measurements especially when the unit is cold started as temperature differences of the unit could be noticeable in the spectra 14 To leave the results screen tap on ...

Page 31: ...een 2 sec and 60 min 3600 sec To exclude any systematic errors a blank sample blank has to be measured at the start of each series of measurements For optimal results it is recommended to turn on the fluidlab and allow the instrument to warm up ahead of performing any spectrometer analysis In general this blank contains the matrix of the sample without corresponding analytes The Kinetics applicati...

Page 32: ...is important to enter the number of steps required for your experiment Steps as well as the time interval Interval between each measurement It is possible to specify a time interval between 2 sec and 60 min 3600 sec The maximum number of steps is limited to 500 The estimated total time of the measurement series will be shown at the bottom of the screen Note The total measuring time is always one t...

Page 33: ...ure 12 Prepare analysis screen of the Kinetics application 12 The device now displays the progress of the time series measurement on the Reaction Kinetics screen Figure 13 13 If you scroll down further a graph showing the extinction value at the pre set wavelength over time is also displayed Every new measurement will be automatically added to this graph Figure 13 14 It is possible to stop the Kin...

Page 34: ... aborted automatically The device automatically saves all the measured values which were performed before the abortion Figure 13 Sample acquisition for kinetics reaction 15 It is possible to view the extinction value at a specific time point by clicking on it directly in the graph Figure 14 Results screen of the Kinetics application ...

Page 35: ...concentration concentration first sample specify the highest concentration of your samples and then indicate the dilution factor and the number of samples you wish to use Note Creating a new curve with concentration If your calibration samples do not have a constant dilution factor it is advisable to create a new calibration curve by means of concentration In this case it makes more sense to enter...

Page 36: ...and save them on the device you can call up the created curves at a later time How quantification works with created curves is described in Chapter 6 2 5 2 6 The Set parameters input screen appears Figure 16 in which you can optionally give the measurement a test name 7 Now enter the mandatory wavelength at which the extinction value of the sample is to be measured 8 Select the appropriate mandato...

Page 37: ...n the correct orientation 11 Start the blank measurement by tapping on the green Measure blank button 12 After a successful blank measurement remove the blank sample from the measuring chamber 13 Now following the prompt on the Prepare analysis screen insert your first sample in the measuring chamber and start the measurement by tapping on the green Continue button 14 After a successful measuremen...

Page 38: ...d concentrations below the graph The green cursor shows the currently selected sample Figure 18 left 20 If you scroll down further you will see a list of all the measured samples with the predefined concentrations and the respective extinction values Figure 18 right Note Activation and deactivation of individual measured values If individual measured values cannot be used for the creation of the c...

Page 39: ...ibration Curve 1 Perform an extinction determination of your choice with your samples 2 Prepare your samples and the respective blank liquid in the cuvettes and make the samples ready for spectrometric measurement with the fluidlab R 300 3 Open the slider on the fluidlab R 300 as described in Chapter 6 2 2 4 Insert sample carrier adapter 02 or 03 as described in Chapter 6 2 2 5 On the home screen ...

Page 40: ...iple saved calibration curves with a long press and then delete them all together You can end the selection mode by tapping on the arrow on the left hand side of the bar If you tab on the pencil the saved curve with its individual points will be shown and can be adjusted 7 You will now go directly to the Prepare Analysis screen There you can optionally enter the Test name for your measurement 8 In...

Page 41: ...hen processed by anvajo s intelligent mathematical reconstruction software which delivers high resolution images In addition machine learning methods such as deep neural networks guarantee accurate counting and classification of the optical outputs with immediate quantitative results 6 3 2 Preparation of the acella Sample Carrier Note acella is a proprietary sample carrier format made of glass sub...

Page 42: ...g the acella adapter and filling the acella sample carrier Left The appropriate adapter for acella is adapter 01 Centre Filling the acella sample carrier with the sample fluid Right Correct orientation of the acella sample carrier when being inserted into the device coloured square on the lower right hand side Warning When filling the sample chamber be careful not to overfill it as sample fluid ma...

Page 43: ...lls after washing The application can also be used very effectively in the preparation of experiments if the previous quantification of the cells is relevant for the test result or for further calculations 1 Prepare your cells as described in the note section above 2 Open the slider measuring chamber cover on the fluidlab R 300 as described in Chapter 6 2 2 3 Insert sample carrier adapter 01 as de...

Page 44: ...tically recognises the different counting chamber heights of the acella 20 50 and 100 and changes the chamber height accordingly in the volume calculation Only these sample carriers are authorised for the fluidlab R 300 Figure 23 left Correct insertion of the acella sample carrier The coloured square must be in the lower right hand corner Figure 24 right Warning if the sample carrier is inserted i...

Page 45: ...he cell image that all objects within the user specified size range were detected and marked by a green box If necessary the cell suspension should be further diluted and a new sample measured Moreover a warning is displayed if there are many cell clumps in the cell suspension that may affect the measurement result Cell clumps are often an indication of poor cell culture quality e g by long incuba...

Page 46: ...ight Display of the cell image in full screen mode 13 The green plus key allows you to go directly to the next measurement with the same parameter setting which is automatically numbered consecutively 14 Tap on the to end the display and after a prompt to save the results in history or discard them Figure 9 ...

Page 47: ...uring chamber cover on the fluidlab R 300 as described in Chapter 6 2 2 3 Insert sample carrier adapter 01 as described in Chapter 6 3 2 4 Fill the acella as described in Chapter 6 3 2 5 On the home screen of the fluidlab R 300 select the Viability application by tapping on it 6 The Set parameters input screen appears Figure 29 in which you can optionally specify the Test name for the measurement ...

Page 48: ...an orange frame Note Histogram for cell size and viability In the histogram Figure 30 right on the Viability screen the cell count is plotted against cell size The orange bars show the distribution of the dead cells and the green bars the distribution of the living cells You can move the cursors to subsequently adjust the object size and discover how many cells of a particular size category have b...

Page 49: ... left Section of a cell image on the results screen Figure 31 right Display of the cell image in full screen mode 14 In full screen mode tap on the to exit the view and return to the Viability screen 15 The green plus key allows you to go directly to the next measurement with the same parameter setting which is automatically numbered consecutively 16 Tap on the to end the display and after a promp...

Page 50: ...lter function allows you to display only those measurements that were carried out with a particular application extinction measurements cell counts quantifications with calibration curves viability measurements kinetics It is also possible via the search functions to display only measurements with a particular test name Figure 33 Filter and sort function in History Figure 34 Editing menu in Histor...

Page 51: ...he Delete history button at the end of the History list can be used 6 5 Quality Assurance Figure 35 Maintenance options 6 5 1 Sensor Status Checking the sensor status is a quality control procedure for maintaining the fluidlab R 300 s measuring functionality and accuracy 1 Open the settings menu of the fluidlab R 300 by tapping the menu icon 2 Tap on the menu item Maintenance 3 Tap on the menu ite...

Page 52: ...calibrated if no detection takes place even if the sample carrier appears to be positioned correctly 1 Open the settings menu of the fluidlab R 300 by tapping the menu icon 2 Tap on the menu item Maintenance 3 Tap on the menu item Refresh Sensor see Figure 35 4 Tap on Start sensor refresh Figure 37 5 The device collects the new sensor data 6 The end of the measurement is indicated by OK Warning Th...

Page 53: ...User Manual fluidlab R 300 54 Edition 202104 Vers 1 2 ...

Page 54: ...ab R 300 to an existing WLAN see Chapter 5 2 2 2 Connect your PC to the same network 3 Open the anvajo datalab desktop client 4 Choose the desired device Note If the device is not automatically shown on the homescreen of your datalab add the device manually by clicking on the button in the upper right corner Type in the hostname fluidlab 000xxx of your fluidlab R 300 which you can find in the syst...

Page 55: ...to export 6 Select the path where the files should be saved 7 Click 7 3 Updates via datalab If it is not possible to connect the fluidlab R 300 to a WLAN you can also update it with a few more steps via the Hotspot and with the help of the datalab 1 Open the anvajo datalab desktop client 2 Connect your fluidlab R 300 via the Hotspot to your PC see Chapter 7 1 2 3 Click in the upper right corner 4 ...

Page 56: ...fluidlab R 300 has been designed in such a way that no special product training is required to operate it 8 2 3 Service Life The fluidlab R 300 is suitable for 24 7 use Portable use is limited by the battery life The 24 7 operating mode requires the external power supply unit to be connected If the battery is low 20 remaining capacity the battery icon on the user interface menu bar of the fluidlab...

Page 57: ...used as intended and that it is connected and operated in accordance with the specifications contained in this user manual anvajo GmbH guarantees a fault free functioning system In the event of any device malfunction or failure attributable to material or manufacturing defects the device will be repaired free of charge within the warranty period The warranty period is one year and commences on the...

Page 58: ...nty 8 5 Cleaning 8 5 1 General The fluidlab R 300 does not require any special form of cleaning Dirt should be removed with a lint free cleaning cloth Cleaning must be carried out in a dry condition Contact with the biohazardous samples may result in infection Hence the components of the device associated with the biohazardous samples are potentially biohazardous Please follow Good Laboratory Prac...

Page 59: ...tant lab coat and approved lab gloves 8 5 3 Cleaning the Sensor and Measuring Chamber The measuring chamber Figure 38 can be cleaned by removing the sample carrier adapter The sample carrier adapters must be cleaned separately We recommend that the measuring chamber should be cleaned daily in order to maintain the device s measuring performance We recommend the following cleaning supplies Swabs fo...

Page 60: ...ntly on the back of the device so that any dust or loose particles fall out 3 With the swabs first clean the sensor surface and then the LED Figure 38 Sensor cleaning process Item No Name Description 1 Sensor For microscopic measurement 2 LED For spectrometer measurement Procedure in the event of heavier contamination e g ingress of fluids 1 First remove the sample carrier adapter and clean it wit...

Page 61: ...th DGUV German Social Accident Insurance Regulation 3 or local equivalent regulation for examination of electrical appliances 8 7 Service Support Please notify the anvajo GmbH Service department or your local authorized distributor or dealer if any deviations are detected on the system Address anvajo GmbH Zwickauer Straße 46 01069 Dresden Tel 49 0 351 854784 00 E mail support anvajo com Web https ...

Page 62: ...ct on human health and on nature The device is equipped with a LiPo battery Batteries require special care in handling and disposal see Chapter 2 Improper disposal may have adverse effects on human health and may harm the environment At the end of its product life cycle the device should be disposed of as electronic waste Please contact the dealer from whom you purchased the device or return it di...

Page 63: ...User Manual fluidlab R 300 64 Edition 202104 Vers 1 2 ...

Page 64: ... 3 7 V 1500 mAh Charging port USB port type C Touch screen Display 3 5 with 320x240px landscape Touch screen Capacitive WLAN Frequency range 2 400 2 4835 GHz IEEE 802 11 b g n Radio channels 13 at IEEE 802 11 b g n 2 4 GHz Transfer rate Realistic transfer rate 35 Mbps IEEE 802 11 b up to 11 Mbps IEEE 802 11 g up to 54 Mbps IEEE 802 11 n up to 300 450 Mbps Security WPA WPA2 Firewall 1 Operating Sys...

Page 65: ...E 106 T1 SK II Degree of protection according to IEC 60529 IP20 IP2X Protection against ingress of solid foreign bodies 12 5 mm IPX0 No protection against ingress of water Dimensions Wx x Dz x Hy fluidlab R 300 128 mm x 94 mm x 33 mm Weight fluidlab R 300 240 g Microscope Resolution Particle size 3 µm 80 µm Spectrometer Frequency band wavelength VIS 2 375 nm 700 nm 3 UV LEDs 365 nm with e 13 mW 20...

Page 66: ... sample carrier 10085 Microscope sample carrier with a chamber height of 100 µm Cleaning swabs 10078 Plastic sticks with cellulose scraper set of 12 Sample carrier adapter 01 acella 10039 Plastic insert for measuring chamber for acellas Sample carrier adapter 02 cuvette 10135 Plastic insert for measuring chamber for scattered light measurements with cuvettes Sample carrier adapter 03 cuvette 10131...

Page 67: ...User Manual fluidlab R 300 68 Edition 202104 Vers 1 2 ...

Page 68: ... directives including any applicable changes thereto in force at the time of this declaration The following standards and technical specifications have been taken into account EN 61010 1 2010 Safety requirements for electrical equipment for measurement control and laboratory use EN 62471 2009 Photobiological safety of lamps and lamp systems EN 61326 1 2013 Electrical equipment for measurement cont...

Page 69: ...User Manual fluidlab R 300 70 Edition 202104 Vers 1 2 ...

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