User Manual fluidlab R-300
48
Edition 202104
Vers.: 1.2
6.3.4
Viability
6.3.4.1
Automatic Viability Measurement
Note
:
Sample preparation of adherent cells and suspension cells - The Viability
application enables you to determine the viability of the cells in the sample without
staining, if they are in suspension.
For adherent cells, this means that they have to
be detached mechanically or chemically from the bottom of the culture vessel
before the analysis. If the cell suspension is so concentrated that cells overlap in the
sample carrier, it should be diluted for a further measurement. Viability is calculated
from the number of living cells in relation to the total cell count. In addition to
viability, the results also list the total number of cells and the number of living and
dead cells. This data evaluation can be used for a variety of experimental designs
and to answer various questions.
1.
Perform a viability experiment of your choice and prepare your cells in
suspension.
2.
Open the slider (measuring chamber cover) on the
fluidlab
R-300
,
as
described in Chapter 6.2.2.
3.
Insert sample carrier adapter 01, as described in Chapter 6.3.2.
4.
Fill the
acella
as described in Chapter 6.3.2.
5.
On the home screen of the
fluidlab
R-300
,
select the
Viability
application by
tapping on it.
6.
The
“Set parameters”
input screen appears (
Figure 29
), in which you can
optionally specify the
Test name
for the measurement and the
Cell type
, as
well as the mandatory
Object size
and the
Dilution
.
Note
:
In the Dilution input field, enter the dilution level for your sample.
The Viability
application uses the dilution factor you have entered to calculate how many cells
are located in your initial solution and shows you the value for the undiluted
solution in the results screen. If you have not performed a dilution, enter a 1:1
dilution in the input field. For a 1:10 dilution, the calculation is based on 1 part
sample and 9 parts dilution solution. In the Cell type input field you can enter
information regarding your samples, e.g. cell type and abbreviations for incubation
times and concentrations.
7.
Confirm what you have entered by tapping on the green tick sign
.
8.
Insert the sample carrier into the slot on the
fluidlab
R-300.
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