anvajo fluidlab R-300, User Manual, Page: 45 / 69

User Manual fluidlab R-300
46
Edition 202104
Vers.: 1.2
Figure 25 (left): “Counted objects” screen of the Cell Count application.
Figure 26 (right): Histogram of cell size in the Cell Count application.
Note : Warning for high cell density or cell cluster – If cell density is very high or if there
are many cell clusters detected in the cell suspension, the number of cells per ml
will be marked in orange in the results screen. By clicking on the orange cell number,
a full screen warning with more information is displayed. If the cell density is very
high, cells may overlap in the sample carrier and may no longer be reliably detected
and counted. We therefore recommend checking the measurement results
critically, and in particular, to check the cell image that all objects within the user-
specified size range were detected and marked by a green box. If necessary, the cell
suspension should be further diluted, and a new sample measured. Moreover, a
warning is displayed if there are many cell clumps in the cell suspension that may
affect the measurement result. Cell clumps are often an indication of poor cell
culture quality, e.g. by long incubation times with trypsin for cell detachment.
7. Start the measurement by tapping on the green tick sign .
8. After the measurement, the results will be displayed in concentration per mL
on the
“Counted objects” screen (Figure 25).
9. Scroll down further to see a section of the cell image.
10. Tap on the double arrow button to enlarge the image (Figure 27, Figure
28).
11. Swipe your index finger across the screen to move to different areas of the
image.
12. In full screen mode, tap on the to exit the view and return to the
“Counted objects” screen.