Operation
30
Edition 02.19
qTOWER
3
84 / qTOWER
3
84 G
7.3
Inserting samples / Starting the PCR analysis
WARNING! Biological hazard!
Take great care when working with potentially infectious material. Wear suitable protective
equipment, e.g. protective gloves.
WARNING
Risk of eye injury!
The rapid heating of the thermal block can cause liquids to boil explosively. Always wear
safety goggles during operation.
CAUTION! Hot surface!
The thermal block, the samples and the heated lid reach high temperatures. There is a risk
of burns during contact.
The qTOWER³ 84 is equipped with a 384 well SBS sample block. This makes it only
suitable
for the use of commercially available PCR plates with 384 wells.
These and
other consumables can be ordered from Analytik Jena AG.
After pipetting the PCR samples into the wells these must be sealed with a transparent
optical adhesive film (sealing film). The optical transparency of the films affects the fluo-
rescence signal directly. Therefore, only use clear adhesive film as offered for real-time
PCR.
1. Prepare a real-time PCR project with complete details about the PCR run, fluores-
cence measurements and the sample layout of the PCR plate (see qPCRsoft384
manual).
2. Open the lid. To do so, press in the red handle on the front until the lock clicks
open. Fold back the upper part of the device.
3. Place the PCR plates onto the thermal block in such a way that well A1 is on the
left-hand side (arrow in the figure below). This position corresponds to the well al-
location in the qPCRsoft384 program.
