Analytik Jena 845-00003-03, User Manual

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Technical Description
Washing
(conti-
nued)
Magnetic particles are subsequently collected again at
the well bottom and any excessive amount is transferred
back into the reagent plate that contains pre-filled
washing buffer. This procedure is several times repeated
using different types of washing buffer. The number of
required washing cycles, the required volume and the
type of washing buffer are conditional on the type of
selected starting material.
5.
Transfer
pipetting
Depending on the particular protocol, the complete con-
tent of a plate is transferred into a new plate during and/
or on completion of a washing routine. This distinctly
improves the quality of nucleic acids already washed. Any
residual amount of lysis debris at the well walls will thus stay
back in the latest washed plate and only those magnetic
particles which have already been washed and are bound
to nucleic acids will be subject to further transportation.
6.
Tip
washing
In order to avoid any carry-over effects of residue poten-
tially accumulating in the tips, the tips are subjected to
rinsing in between certain washing steps.
7.
Ethanol
removal
To remove residue of ethanol inside the wells, a drying
workstep follows. This workstep consists in the heating
plate of the heating magnet module being turned on and
pressed against the bottom of the plate.
8.
Elution
Elution buffer is supplied. This is followed by heating of
the mixture and homogenization by performing continu-
ous pipetting-on and pipetting-off cycles. In the process
nucleic acids get detached from the magnetic particles.
As part of an elution workstep magnetic particles are
again collected at the well bottom and the mixture is
subsequently transferred to a new plate where the re-
maining magnetic particles are once more collected at
the well bottom.
9.
Eluate
transfer
Once all magnetic particle residues have gathered at the
well bottom, the eluate is transferred into the elution
plate. The software screen reports completion of the proto-
col sequence and the elution plate which is located in the
left carriage position can now be retrieved from the system
(after opening of the left door).
Figure Step Explanation