Analytik Jena 845-00003-03, User Manual

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30 2015
Technical Description
4.4 Mode of Operation/Functionality
Depending on the type and features of selected starting material, a given lysis process
may have to be performed inside the system (internal process) or outside the system
(external process) in manual mode.
Refer to the user manual of a given extraction kit for a detailed description of lysis
working steps/instructions for the corresponding type of starting material.
Table 6: Protocol sequence
Figure Step Explanation
1.
Lysis
(internal) For internal lysis, the necessary reagents are at first added
to the samples in a sample plate and the sample plate is
placed onto the left carriage position. This is followed by
motion to transfer the plate into its working position
below the pipetting head. In the majority of cases, the
heating plate is then pressed against the well bottoms in
order to process thermal pulpings. Continuous mixing
throughout this working step will help the lysis process.
Lysis
(external) For external lysis, a sample plate is equally placed onto the
left carriage position. The automatic protocol routine
begins with the workstep of binding.
2.
Binding The procedure of binding nucleic acids to the magnetic
particles begins with the addition of binding buffer. Once
the binding buffer has been transferred, a homogeneous
mixture of the various involved components is achieved by
way of pipetting-on and pipetting-off steps performed in
quick succession.
3.
Collecting To collect the magnetic particles at the plate’s well bot-
tom, the heating magnet module below the plate moves
into place at the plate. It moves exactly as far as necessary
for the magnets to make direct contact with the well bot-
tom through the heating plate. Any excessive binding buffer
can thus be pipetted off and discarded.
4.
Washing Any residual amount of magnetic particles at the well bot-
tom can now be solved in a washing buffer. Repeated/mul-
tiple pipetting-on and pipetting-off cycles warrant proper
mixing.