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Agilent 1290 Infinity Binary Pump User Manual
4
Using the Pump
Priming the Pump
Priming the Pump
When the solvents have been exchanged or the pumping system has been
turned off for a certain time (for example, overnight) oxygen will re-diffuse
into the solvent channel between the solvent reservoir, vacuum degassing unit
(when available in the system) and the pump. Solvents containing volatile
ingredients will slightly lose these. Therefore priming of the pumping system
is required before starting an application.
1
Initiate a purge in the controlling software with a Purge flow set to
3 – 5 ml/min per channel.
2
Flush all tubes with at least 30 ml of solvent.
Table 7
Choice of Priming Solvents for Different Purposes
Activity
Solvent
Comments
After an installation
When switching between reverse phase and
normal phase (both times)
Isopropanol
Isopropanol
Best solvent to flush air out of the system
Isopropanol is miscible with both normal phase
and reverse phase solvents.
After an installation
Ethanol or Methanol
Alternative to Isopropanol (second choice) if no
Isopropanol is available
To clean the system when using buffers
After a solvent change
Bidistilled water
Bidistilled water
Best solvent to re-dissolve buffer cristals
Best solvent to re-dissolve buffer cristals
Before turning off system for an extended
period of time
Organic or 10 %
isopropanol in water
N O T E
The pump should never be used for priming empty tubings (never let the pump run dry). Use
a syringe to draw enough solvent for completely filling the tubings to the pump inlet before
continuing to prime with the pump.
