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Agilent 1260 Infinity VWD User Manual

Agilent Technologies

4
Using the Detector

Leak and Waste Handling

Setting up an Analysis

Before Using the System

Requirements and Conditions

Optimization of the System

Preparing the HPLC System

Running the Sample and Verifying the Results

Special Settings of the Detector

Control Settings

Online Spectra

Scanning with the VWD

Analog Output Settings

Special Setpoints

This chapter provides information on how to set up the detector for an analysis
and explains the basic settings.

Summary of Contents for G1314B

Page 1: ...Agilent Technologies Agilent 1260 Infinity Variable Wavelength Detector User Manual...

Page 2: ...the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in...

Page 3: ...uirements and Specifications This chapter gives information on environmental requirements physical and performance specifications 3 Installing the Detector This chapter provides information on unpacki...

Page 4: ...t functions 9 Maintenance This chapter provides general information on maintenance and repair of the detector 10 Parts and Materials for Maintenance This chapter provides information on parts for main...

Page 5: ...tack Configuration 34 Installation Information on Leak and Waste Handling 39 Installing the Detector 43 Flow Connections to the Detector 46 4 Using the Detector 49 Leak and Waste Handling 50 Setting u...

Page 6: ...ft and Noise Test 110 Quick Noise Test 111 Dark Current Test 112 Holmium Oxide Test 114 9 Maintenance 117 Introduction to Maintenance 118 Warnings and Cautions 119 Overview of Maintenance 121 Cleaning...

Page 7: ...re Flow Cell 10 mm 14 L 146 Cuvette Holder 148 Leak Parts 149 Kits 150 11 Identifying Cables 151 Cable Overview 152 Analog Cables 154 Remote Cables 156 BCD Cables 159 CAN LAN Cables 161 RS 232 Cable K...

Page 8: ...ents 13 Appendix 191 General Safety Information 192 Batteries Information 195 Radio Interference 196 Sound Emission 197 UV Radiation 198 Solvent Information 199 Declaration of Conformity for HOX2 Filt...

Page 9: ...12 G1314B C Lamp 13 Source Lens Assembly 13 Entrance Slit Assembly 13 Filter Assembly 13 Mirror Assemblies M1 and M2 14 Grating Assembly 14 Beam Splitter Assembly 14 Photo Diodes Assemblies 15 Photo D...

Page 10: ...lowest detection limit over a wavelength range of 190 to 600 nm optional flow cell cartridges standard 10 mm 14 L high pressure 10 mm 14 L micro 5 mm 1 L semi micro 6 mm 5 L are available and can be u...

Page 11: ...in the cell in which UV absorption takes place and the intensity is converted to an electrical signal by means of the sample photodiode Part of the light is directed to the reference photodiode by th...

Page 12: ...l Table 1 Flow Cell Data STD Semi micro Micro High Pressure Maximum pressure 40 4 40 4 120 12 400 40 bar Path length 10 conical 6 conical 3 conical 10 conical mm Volume 14 5 2 14 L Inlet i d 0 25 0 17...

Page 13: ...uses it onto the entrance slit Entrance Slit Assembly The entrance slit assembly has an exchangeable slit The standard one has a 1 mm slit For replacement and calibration purposes to optimize the alig...

Page 14: ...nd M2 The beam adjustable is vertically and horizontally Both mirrors are identical Grating Assembly The grating separates the light beam into all its component wavelengths and reflects the light onto...

Page 15: ...e sample diode assembly is located on the left side of the optical unit The reference diode assembly is located in the front of the optical unit Photo Diode ADC analog to digital converter The photo d...

Page 16: ...e Variable Wavelength Detector System Overview System Overview Leak and Waste Handling The 1200 Infinity Series has been designed for safe leak and waste handling It is important that all security con...

Page 17: ...lent 1260 Infinity VWD User Manual 17 Introduction to the Variable Wavelength Detector 1 System Overview Figure 4 Leak and waste handling concept overview typical stack configuration as an example 6 7...

Page 18: ...lso leakages on internal parts e g the detector s flow cell The leak sensor in the leak pan stops the running system as soon as the leak detection level is reached The leak pan s outlet port 3 A guide...

Page 19: ...ologies 2 Site Requirements and Specifications Site Requirements 20 Physical Specifications 24 Performance Specifications 25 Specification Conditions 29 This chapter gives information on environmental...

Page 20: ...ernally accessible fuses because automatic electronic fuses are implemented in the power supply WARNING Instrument is partially energized when switched off The power supply still uses some power even...

Page 21: ...sily reached and unplugged Provide sufficient space behind the power socket of the instrument to unplug the cable WARNING Absence of ground connection or use of unspecified power cord The absence of g...

Page 22: ...imately 8 cm 3 1 inch in the rear for air circulation and electric connections If the bench should carry an Agilent 1200 Infinity Series system make sure that the bench is designed to bear the weight...

Page 23: ...tter drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1 C h 1...

Page 24: ...nches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption 220 VA 85 W 290 BTU Maximum Ambient operating temperature 0 55 C 32 131 F Ambient non operating...

Page 25: ...254 nm See Specification Conditions on page 29 Linearity 2 AU 5 upper limit See Specification Conditions on page 29 Wavelength accuracy 1 nm Self calibration with deuterium lines verification with ho...

Page 26: ...e Extensive diagnostics error detection and display through Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance ar...

Page 27: ...age 29 Linearity 2 AU 5 upper limit See Specification Conditions on page 29 Wavelength accuracy 1 nm Self calibration with deuterium lines verification with holmium oxide filter Maximum data rate 55 H...

Page 28: ...or detection and display through Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas GLP features Early main...

Page 29: ...ift Better drift performance depends on better control of the temperature fluctuations To realize the highest performance minimize the frequency and the amplitude of the temperature changes to below 1...

Page 30: ...30 Agilent 1260 Infinity VWD User Manual 2 Site Requirements and Specifications Performance Specifications...

Page 31: ...ector Accessory Kit Contents 33 Optimizing the Stack Configuration 34 One Stack Configuration 34 Two Stack Configuration 37 Installation Information on Leak and Waste Handling 39 Installing the Detect...

Page 32: ...tempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged Notify your Agilent sales and service office about the damage An Agilent ser...

Page 33: ...arts to your local Agilent Technologies sales and service office Detector Accessory Kit Contents The G1314B C VWD is shipped with Accessory kit G1314 68755 see Accessory Kit on page 150 Table 5 Variab...

Page 34: ...mance by installing the following configurations These configurations optimize the system flow path ensuring minimum delay volume One Stack Configuration Ensure optimum performance by installing the m...

Page 35: ...Manual 35 Installing the Detector 3 Optimizing the Stack Configuration Figure 5 Recommended Stack Configuration for 1260 Infinity Front View 9ZiZXidg KVXjjb YZ VhhZg Ejbe 6jidhVbeaZg 8dajbc XdbeVgibZc...

Page 36: ...Optimizing the Stack Configuration Figure 6 Recommended Stack Configuration for 1260 Infinity Rear View 68 edlZg 86C 7jh XVWaZ id 86C 7jh XVWaZ 6cVad YZiZXidg h cVa dg djiejih eZg YZiZXidg GZbdiZ XVW...

Page 37: ...s Some users prefer the lower height of this arrangement even without the autosampler thermostat A slightly longer capillary is required between the pump and autosampler See Figure 7 on page 37 and Fi...

Page 38: ...ing the Detector Optimizing the Stack Configuration Figure 8 Recommended Two Stack Configuration for 1260 Infinity Rear View A6C id Xdcigda hd ilVgZ 86C 7jh XVWaZ id chiVci E adi 86C 7jh XVWaZ GZbdiZ...

Page 39: ...fety gloves and protective clothing as described in the material handling and safety data sheet supplied by the vendor and follow good laboratory practice The volume of substances should be reduced to...

Page 40: ...gilent 1260 Infinity VWD User Manual 3 Installing the Detector Installation Information on Leak and Waste Handling Figure 9 Leak and waste handling overview typical stack configuration as an example 6...

Page 41: ...m manual 1 Solvent cabinet 2 Leak pan 3 Leak pan s outlet port A leak funnel B and corrugated waste tube C 4 Waste tube of the sampler s needle wash 5 Condense drain outlet of the autosampler cooler 6...

Page 42: ...42 Agilent 1260 Infinity VWD User Manual 3 Installing the Detector Installation Information on Leak and Waste Handling Figure 10 Warning label illustration for correct waste tubing...

Page 43: ...k or on the bench in a horizontal position Parts required p n Description 1 Detector 1 Power cord 1 LAN cable cross over or twisted pair network cable 1 Agilent ChemStation or other control software 1...

Page 44: ...ct the LAN connection to the LAN interface board in the detector 7 Connect the analog cable optional 8 Connect the APG remote cable optional for non Agilent 1260 Infinity instruments HiVijh cY XVidg g...

Page 45: ...e detector is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The detector is turned OFF when the line power switch is protruding and the green light is OFF...

Page 46: ...ents samples and reagents can hold health and safety risks When working with these substances observe appropriate safety procedures for example by wearing goggles safety gloves and protective clothing...

Page 47: ...ector uses a metal plate loose the screws of the flow cell dummy plate by turning each screw one turn Then unscrew the screws completely This is required to avoid any problem with helicoil insert in t...

Page 48: ...bled fitting of the capillary to the inlet connector and connect the other end of the capillary to the column 6 Connect the PEEK waste capillary to the outlet connector 7 Establish a flow and observe...

Page 49: ...Conditions 53 Optimization of the System 55 Preparing the HPLC System 56 Running the Sample and Verifying the Results 65 Special Settings of the Detector 66 Control Settings 66 Online Spectra 67 Scan...

Page 50: ...quired for the analysis Do not operate the instrument in an explosive atmosphere Never exceed the maximal permissible volume of solvents 6 L in the solvent cabinet Do not use bottles that exceed the m...

Page 51: ...n Observe recommendations on the use of solvents in chapter Solvents in the pump s reference manual Priming and Purging the System When the solvents have been exchanged or the pumping system has been...

Page 52: ...tem Best solvent to flush air out of the system After an installation Ethanol or Methanol Alternative to Isopropanol second choice if no Isopropanol is available To clean the system when using buffers...

Page 53: ...Table 7 What you will need Agilent 1200 Infinity Series system Pump plus degassing Autosampler Detector standard flow cell installed Degasser optional Column Compartment optional Agilent ChemStation o...

Page 54: ...ngle injection of the isocratic test standard is made under the conditions given in Table 8 on page 54 Table 8 Conditions Flow 1 5 mL min Stoptime 8 min Solvent 100 30 water 70 Acetonitrile Temperatur...

Page 55: ...c conditions Variations in solvent quality column packing standard concentration and column temperature will all have a potential effect on peak retention and response Figure 13 Typical Chromatogram w...

Page 56: ...rn on the modules 3 Start the Agilent ChemStation software If the pump autosampler thermostatted column compartment and detector are found the Agilent ChemStation screen should look like shown in Figu...

Page 57: ...Turn on the detector lamp pump and autosampler by clicking the System On button or the buttons below the module icons on the graphical user interface GUI After some time the pump thermostatted column...

Page 58: ...le baseline see example in Figure 16 on page 58 Figure 16 Stabilization of Baseline 7 For the isocratic pump fill the solvent bottle with the mixture of HPLC grade bi distilled water 30 and acetonitri...

Page 59: ...tor 4 Setting up an Analysis 8 Click on the Load Method button select DEF_LC M and press OK Alternatively double click on the method in the method window The default LC method parameters are transferr...

Page 60: ...is 9 Click on the module icons Figure 18 on page 60 and open the Setup of these modules Figure 19 on page 61 shows the detector settings do not change the detector parameters at this time Figure 18 Op...

Page 61: ...peaks in the chromatogram see Peakwidth Settings on page 71 time table for programmable actions during the run Zero Offset Limits 1 99 in steps of 1 Attenuation Limits 0 98 4000 mAU at discrete values...

Page 62: ...10 minutes for equilibration 12 Click the button and select Change to open the Signal Plot information Select the Pump Pressure and the VWD A Signal 254 as signals Change the Y range for the VWD to 1...

Page 63: ...nals Pressing Adjust the signals can be reset to the offset value and Balance would do a balance on the detector Figure 21 Online Plot Window 13 If both baselines are stable set the Y range for the de...

Page 64: ...tem RunControl Sample Info and enter information about this application Figure 22 on page 64 Press OK to leave this screen Figure 22 Sample Information 15 Fill the content of an isocratic standard sam...

Page 65: ...select the menu item RunControl Run Method 2 This will start the modules and the online plot on the Agilent ChemStation will show the resulting chromatogram Figure 23 Chromatogram with Isocratic Test...

Page 66: ...rn on and off of UV lamp At Power On automatic lamp on at power on Error Method take error method or current method in case of an error Analog Output Range can be set to either 100 mV or 1 V full scal...

Page 67: ...Spectra 1 To view the online spectra select Online Spectra Figure 25 Online Spectra Window 2 Change the absorbance and wavelength range according your needs NOTE This online spectrum is taken during a...

Page 68: ...nts to stabilize the concentration 5 Select from the menu Instrument More VWD Sample Scan A sample scan is taken in the range defined under Special Setpoints on page 70 and the Online Spectra window s...

Page 69: ...trol 2 To change the offset and the attenuation select VWD Signal More 3 Change the values if required Figure 26 Analog Output Settings Analog Output Range can be set to either 100 mV or 1 V full scal...

Page 70: ...tion if for example your solvent gradient produces a decreasing baseline absorbance and for GPC analyses Limits 100 4000 mAU Signal Polarity can be switched to negative if required Enable analysis whe...

Page 71: ...ram The peak width sets the optimum response time for your detector The peak detector ignores any peaks that are considerably narrower or wider than the peak width setting The response time is the tim...

Page 72: ...e Hz 0 005 0 12 13 74 0 005 0 12 13 74 0 01 0 25 13 74 0 025 0 5 13 74 0 05 1 0 6 87 0 10 2 0 3 43 0 20 4 0 1 72 0 40 8 0 0 86 Table 10 Peak Width Response Time Data Rate G1314C VWD SL Peak Width min...

Page 73: ...gilent Technologies 5 How to optimize the detector Optimizing the Detector Performance 74 Match the Flow Cell to the Column 75 Set the Detector Parameters 78 This chapter gives hints on how to select...

Page 74: ...imizing the Detector Performance The detector has a variety of parameters that can be used to optimize performance The information below will guide you on how to get the best detector performance Foll...

Page 75: ...to the Column Figure 30 on page 75 recommends the flow cell that matches the column used If more than one selection is appropriate use the larger flow cell to get the best detection limit Use the sma...

Page 76: ...n the example from 5 14 L Typically this causes more peak dispersion As demonstrated this did not affect the resolution in the gradient separation in the example shown below As a rule of thumb the flo...

Page 77: ...tails of the Agilent 1200 Infinity Series Variable Wavelength Detector flow cells 6WhdgWVcXZ I bZ b c 6cVanh h d eZhi X YZ hiVcYVgY bb dei XVa eVi aZc i bb dei XVa eVi aZc i Table 11 Correction factor...

Page 78: ...Settings on page 71 2 Choose the sample wavelength at a longer wavelength than the cut off wavelength of the mobile phase at a wavelength where the analytes have strong absorptivity if you want to ge...

Page 79: ...and Diagnostics Overview of the Detector s Indicators and Test Functions 80 Status Indicators 81 Power Supply Indicator 81 Module Status Indicator 82 Available Tests versus Interfaces 83 Agilent Lab...

Page 80: ...the user interface For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided Test Functions A series...

Page 81: ...f the detector The lower left indicates the power supply status the upper right indicates the detector status Figure 32 Location of Status Indicators Power Supply Indicator The power supply indicator...

Page 82: ...dicator is red An error condition indicates the module has detected an internal problem which affects correct operation of the module Usually an error condition requires attention e g leak defective i...

Page 83: ...may not include any maintenance test functions Table 12 Available Tests versus Interface Interface Test Lab Advisor ChemStation Instant Pilot G4208A Wavelength Verification Re calibration Calibration...

Page 84: ...tic capabilities calibration procedures and maintenance routines for all the maintenance routines The Agilent Lab Advisor software also allows users to monitor the status of their LC instruments The E...

Page 85: ...r 93 Detector Error Messages 94 UV lamp no current 94 UV lamp no voltage 94 Ignition Failed 95 No heater current 96 Wavelength calibration setting failed 97 Wavelength holmium check failed 98 Grating...

Page 86: ...fication all LEDs get red and the run will be stopped Depending on the module type this stop is implemented differently For example for a pump the flow will be stopped for safety reasons For a detecto...

Page 87: ...ested actions 1 The analysis was completed successfully and the timeout function switched off the module as requested Check the logbook for the occurrence and source of a not ready condition Restart t...

Page 88: ...of starting the analysis If a not ready condition is still present on the remote line after one minute the error message is generated Probable cause Suggested actions 1 Leak detected in another modul...

Page 89: ...uments connected to the remote line Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis 2 Defective remote cable Exchange the remote cable...

Page 90: ...it The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current...

Page 91: ...on the main board in the module has failed open circuit The resistance across the temperature compensation sensor NTC on the main board is dependent on ambient temperature The change in resistance is...

Page 92: ...ance across the sensor falls below the lower limit the error message is generated Fan Failed Error ID 0068 The cooling fan in the module has failed The hall sensor on the fan shaft is used by the main...

Page 93: ...cause Suggested actions 1 Fan cable disconnected Please contact your Agilent service representative 2 Defective fan Please contact your Agilent service representative 3 Defective main board Please co...

Page 94: ...tage Error ID 7451 The lamp anode voltage is missing The processor continually monitors the anode voltage across the lamp during operation If the anode voltage falls below the lower limit the error me...

Page 95: ...able cause Suggested actions 1 Defective or non Agilent lamp Exchange the lamp 2 Defective power supply Please contact your Agilent service representative 3 Defective main board Please contact your Ag...

Page 96: ...rror message is generated Probable cause Suggested actions 1 Lamp disconnected Ensure the lamp is connected 2 Ignition started without the top foam in place Please contact your Agilent service represe...

Page 97: ...ctions 1 Lamp is OFF Switch on the lamp 2 Incorrect flow cell installation Ensure the flow cell is installed correctly 3 Flow cell contamination or air bubbles Clean replace flow cell windows or remov...

Page 98: ...12 7813 7814 7815 7816 The motor test has failed During the motor tests the detector moves the motor to the end position while monitoring the end position sensor If the end position is not found the e...

Page 99: ...de is performed If the emission line is more than 3 nm away from 656 nm the error message is generated Probable cause Suggested actions 1 Motor is not connected Please contact your Agilent service rep...

Page 100: ...sity decrease is not detected the error message is generated ADC Hardware Error Error ID 7830 7831 A D Converter hardware is defective Probable cause Suggested actions 1 Motor is not connected Please...

Page 101: ...place If the foam is removed while the lamps are on or if an attempt is made to switch on for example the lamps with the foam removed the lamps are switched off and the error message is generated Prob...

Page 102: ...102 Agilent 1260 Infinity VWD User Manual 7 Error Information Detector Error Messages...

Page 103: ...Intensity Test Failed 105 Cell Test 106 Wavelength Verification Calibration 108 ASTM Drift and Noise Test 110 Quick Noise Test 111 Dark Current Test 112 Dark Current Test Failed 113 Holmium Oxide Tes...

Page 104: ...n the flow cell The shape of the intensity spectrum is primarily dependent on the lamp grating and diode characteristics Therefore intensity spectra will differ slightly between instruments Figure 33...

Page 105: ...b Advisor Intensity Test Failed Probable cause Suggested actions 1 Empty flow cell Ensure the flow cell is filled with water 2 Flow cell windows dirty Repeat the test with the flow cell removed If the...

Page 106: ...o real limit The reason is that it depends on the position alignment of the reference side beam splitter reference slit reference diode Therefore the reference side value can be higher smaller than th...

Page 107: ...1260 Infinity VWD User Manual 107 Test Functions 8 Cell Test Figure 34 Cell Test with Lab Advisor Checking the Photocurrent with the Instant Pilot Figure 35 Checking the Photocurrent with the Instant...

Page 108: ...lmium lines are at 360 8 nm 418 5 nm and 536 4 nm When the lamp is turned ON the 656 nm emission line position of the deuterium lamp is checked automatically The Wavelength Verification Calibration is...

Page 109: ...Infinity VWD User Manual 109 Test Functions 8 Wavelength Verification Calibration Wavelength Verification Calibration with Agilent Lab Advisor Figure 36 Wavelength Verification and Calibration Agilent...

Page 110: ...oise Test The ASTM Drift and Noise test determines the detector noise over a period of 20 min The test is done with HPLC grade water flowing through the flow cell at 1 mL min On completion of the test...

Page 111: ...ximum amplitude for all random variations of the detector signal of frequencies greater than one cycle per hour The noise is determined for 5 one minute intervals and is based on the accumulated peak...

Page 112: ...age current from the sample and reference circuits The test is used to check for defective sample or reference diodes or ADC circuits which may cause non linearity or excessive baseline noise During t...

Page 113: ...Failed Probable cause Suggested actions 1 Defective sample or reference diode Please contact your Agilent service representative 2 Defective sample or reference ADC board Please contact your Agilent s...

Page 114: ...Diagnosis VWD Holmium Spectrum Test The test uses the following holmium maxima 360 8 nm 418 5 nm 536 4 nm When to do the Test after recalibration as part of the Operational Qualification Performance V...

Page 115: ...Agilent 1260 Infinity VWD User Manual 115 Test Functions 8 Holmium Oxide Test Running the test with Agilent Lab Advisor Figure 40 Holmium Test with Agilent Lab Advisor...

Page 116: ...Recalibrate the detector 2 Dirty or defective flow cell Repeat the test with the flow cell removed If the test is OK exchange the flow cell components 3 Dirty or defective holmium oxide filter Run the...

Page 117: ...nce 121 Cleaning the Module 122 Exchanging a Lamp 123 Exchanging a Flow Cell 126 Repairing the Flow Cells 128 Using the Cuvette Holder 130 Correcting Leaks 132 Replacing Leak Handling System Parts 133...

Page 118: ...ance Introduction to Maintenance Introduction to Maintenance The module is designed for easy maintenance Maintenance can be done from the front with module in place in the system stack NOTE There are...

Page 119: ...ed by the vendor and follow good laboratory practice The volume of substances should be reduced to the minimum required for the analysis Do not operate the instrument in an explosive atmosphere WARNIN...

Page 120: ...he products failure to comply with procedures in Agilent product user guides or use of the products in violation of applicable laws rules or regulations Use your Agilent products only in the manner de...

Page 121: ...drift exceeds your application limits or lamp does not ignite A VWD test should be performed after replacement Flow cell exchange If application requires a different flow cell type A VWD test should...

Page 122: ...se a soft cloth slightly dampened with water or a solution of water and mild detergent WARNING Liquid dripping into the electronic compartment of your module can cause shock hazard and damage the modu...

Page 123: ...he lamp OFF WARNING Injury by touching hot lamp If the detector has been in use the lamp may be hot If so wait for lamp to cool down NOTE If you want to use the Agilent DAD lamp instead of the VWD lam...

Page 124: ...nance Exchanging a Lamp 1 Press the release buttons and remove the front cover to have access to the lamp area 2 Unscrew the lamp cover and remove it 3 Unscrew disconnect and replace the lamp Insert f...

Page 125: ...Replace the front cover Next Steps 6 Reset the lamp counter as described in the User Interface documentation 7 Turn the lamp ON 8 Give the lamp more than 10 min to warm up 9 Perform Wavelength Verifi...

Page 126: ...Parts required p n Description 1 G1314 60186 Standard flow cell 10 mm 14 L 40 bar with RFID tag OR 1 G1314 60187 Micro flow cell 3 mm 2 L 120 bar with RFID tag OR 1 G1314 60183 Semi micro flow cell 6...

Page 127: ...flow cell completely into the slot and tighten the cell screws both parallel until the mechanical stop Reconnect the inlet and outlet capillaries to the flow cell 5 Replace the front cover Next Steps...

Page 128: ...lary connections Wrench 4 mm hexagonal Toothpick Parts required Description See Standard Flow Cell 10 mm 14 L on page 139 See Micro Flow Cell 3 mm 2 L on page 142 See Micro Flow Cell 5 mm 1 L only for...

Page 129: ...rt the conical springs Make sure the conical springs point towards the window Otherwise tightening the cell screw might break the window Figure 41 Orientation of conical springs 5 Screw the cell screw...

Page 130: ...it for example National Institute of Standards Technology NIST holmium oxide solution standard can be fixed in it This can be used for wavelength verifications When If your own standard should be use...

Page 131: ...vette with the sample into the holder The clear side of the cuvette must be visible 4 Replace the bracket and fix the cuvette Next Steps 5 Install the cuvette holder in the instrument 6 Perform your W...

Page 132: ...3 Observe the capillary connections and the flow cell area for leaks and correct if required 4 Replace the front cover Figure 42 Drying the Leak Sensor When If a leakage has occurred in the flow cell...

Page 133: ...tion 4 Replace the leak funnel and or the tubing 5 Insert the leak funnel with the tubing in its position 6 Insert the leak funnel into the leak funnel holder 7 Replace the front cover Figure 43 Repla...

Page 134: ...nterface board BCD with external contacts and BCD outputs OR 1 G1369B or G1369 60002 Interface board LAN OR 1 G1369C or G1369 60012 Interface board LAN 1 Install the ESD strap Move the power lock acro...

Page 135: ...nstallation of newer firmware might be necessary if a newer version solves problems of older versions or to keep all systems on the same validated revision The installation of older firmware might be...

Page 136: ...Specific Information Table 14 Module Specific Information G1314B C G1314B VWD G1314C VWD SL Initial firmware A 06 02 A 06 02 Compatibility with 1100 1200 series modules yes all modules should have th...

Page 137: ...of Maintenance Parts 138 Standard Flow Cell 10 mm 14 L 139 Micro Flow Cell 5 mm 1 L only for support 140 Micro Flow Cell 3 mm 2 L 142 Semi micro Flow Cell 6 mm 5 L 144 High Pressure Flow Cell 10 mm 1...

Page 138: ...rnal contacts and BCD outputs G1369C or G1369 60012 Interface board LAN G4208 67001 Instant Pilot G4208A requires firmware B 02 08 or above G1314 60100 Deuterium lamp G1314 60186 Standard flow cell 10...

Page 139: ...des 2x Gasket 1 2x Gasket 2 2x Window Quartz 1 G1314 65062 Cell screw kit 2 79853 29100 Conical spring kit 10 pk 3 G1314 65066 Ring 2 kit IN small hole i d 1 mm PEEK 2 pk 4 G1314 65064 Gaskets 2 IN sm...

Page 140: ...o flow cell 5 mm 1 L 40 bar 5021 1823 Capillary column detector SST 400 mm lg 0 12 mm i d 1 G1314 20047 Cell screw G1314 65052 Cell kit micro comprises two windows two gaskets 1 and two gaskets 2 2 79...

Page 141: ...Infinity VWD User Manual 141 Parts and Materials for Maintenance 10 Micro Flow Cell 5 mm 1 L only for support Figure 45 Micro Flow Cell 5 mm 1 L 40 bar 8Zaa hXgZl 8dc XVa heg c h G c HHI Vh Zi L cYdl...

Page 142: ...Window holder 5 1000 0488 Quartz window 6 G1315 68710 Gasket FRONT PTFE 1 3 mm hole inlet side 12 pk 7 79883 68702 Gasket BACK PTFE 1 8 mm hole outlet side 12 pk 8 G1314 44010 Clip for RFI ID tag 9 05...

Page 143: ...ty VWD User Manual 143 Parts and Materials for Maintenance 10 Micro Flow Cell 3 mm 2 L Figure 46 Micro Flow Cell m m L cYdl hXgZl LVh Zgh 8dbegZhh dc lVh Zg L cYdl daYZg FjVgio l cYdl Vh Zi gdci Vh Zi...

Page 144: ...apillary column detector SST 400 mm lg 0 12 mm i d 1 G1314 20047 Cell screw G1314 65056 Semi micro cell kit includes two quartz windows one gasket 1 one 2 and two PTFE gaskets 2 79853 29100 Conical sp...

Page 145: ...anual 145 Parts and Materials for Maintenance 10 Semi micro Flow Cell 6 mm 5 L Figure 47 Semi micro Flow Cell m m 8Zaa hXgZl 8dc XVa heg c h G c HHI EI Vh Zi FjVgio l cYdl HZb b Xgd Vh Zi HZb b Xgd Vh...

Page 146: ...tion G1314 60182 High pressure flow cell 10 mm 14 L 400 bar with RFID tag G1315 87311 Capillary ST 0 17 mm x 380 mm S S 1 G1314 20047 Cell screw G1314 65054 Cell kit Agilent comprises two windows two...

Page 147: ...ent 1260 Infinity VWD User Manual 147 Parts and Materials for Maintenance 10 High Pressure Flow Cell 10 mm 14 L Figure 48 High Pressure Flow Cell 8Zaa hXgZl G c E FjVgio l cYdl Vh Zi 6EIDC G 9 8a e HX...

Page 148: ...Manual 10 Parts and Materials for Maintenance Cuvette Holder Cuvette Holder For information the use of the cuvette holder refer to Using the Cuvette Holder on page 130 Figure 49 Cuvette Holder p n Des...

Page 149: ...als for Maintenance 10 Leak Parts Leak Parts Figure 50 Leak Parts Item p n Description 3 5041 8388 Leak funnel 4 5041 8389 Leak funnel holder 5 5041 8387 Tube clip 6 5062 2463 Corrugated tubing PP 6 5...

Page 150: ...e of the module Accessory Kit Accessory kit G1314 68755 contains some accessories and tools needed for installation and repair of the module p n Description 0100 1516 Fitting male PEEK 2 pk 5062 8535...

Page 151: ...gies 11 Identifying Cables Cable Overview 152 Analog Cables 154 Remote Cables 156 BCD Cables 159 CAN LAN Cables 161 RS 232 Cable Kit 162 External Contact Cable 163 This chapter provides information on...

Page 152: ...dule to 3394 6 integrators 35900 60750 Agilent 35900A A D converter 01046 60105 Analog cable BNC to general purpose spade lugs p n Description 03394 60600 Agilent module to 3396A Series I integrators...

Page 153: ...ion 5023 0202 Twisted pair network cable shielded 7 m for point to point connection p n Description G1103 61611 External contact cable Agilent module interface board to general purposes p n Descriptio...

Page 154: ...Agilent modules The other end depends on the instrument to which connection is being made Agilent Module to 3394 6 Integrators Agilent Module to BNC Connector p n 35900 60750 Pin 3394 6 Pin Agilent mo...

Page 155: ...lent 1260 Infinity VWD User Manual 155 Identifying Cables 11 Analog Cables Agilent Module to General Purpose p n 01046 60105 Pin Pin Agilent module Signal Name 1 Not connected 2 Black Analog 3 Red Ana...

Page 156: ...grators Agilent Module to 3396 Series II 3395A Integrators Use the cable Agilent module to 3396A Series I integrators 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints S...

Page 157: ...ay Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected p n 5061 3378 Pin 35900 A...

Page 158: ...Module to General Purpose p n 01046 60201 Wire Color Pin Agilent module Signal Name Active TTL White 1 Digital ground Brown 2 Prepare run Low Gray 3 Start Low Blue 4 Shut down Low Pink 5 Not connecte...

Page 159: ...to be connected to Agilent Module to General Purpose p n G1351 81600 Wire Color Pin Agilent module Signal Name BCD Digit Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 Yellow 4 BCD 4 10 Black 5 B...

Page 160: ...ng Cables BCD Cables Agilent Module to 3396 Integrators p n 03396 60560 Pin 3396 Pin Agilent module Signal Name BCD Digit 1 1 BCD 5 20 2 2 BCD 7 80 3 3 BCD 6 40 4 4 BCD 4 10 5 5 BCD0 1 6 6 BCD 3 8 7 7...

Page 161: ...d to Agilent modules CAN or LAN connectors CAN Cables LAN Cables p n Description 5181 1516 CAN cable Agilent module to module 0 5 m 5181 1519 CAN cable Agilent module to module 1 m p n Description 502...

Page 162: ...232 cable 2 m RS232 61601 RS 232 cable 2 5 m Instrument to PC 9 to 9 pin female This cable has special pin out and is not compatible with connecting printers and plotters It s also called Null Modem...

Page 163: ...eneral purpose Agilent Module Interface Board to general purposes p n G1103 61611 Color Pin Agilent module Signal Name White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT...

Page 164: ...164 Agilent 1260 Infinity VWD User Manual 11 Identifying Cables External Contact Cable...

Page 165: ...ormation ALL 173 Rear view of the module 174 Interfaces 175 Interfaces Overview 178 Setting the 8 bit Configuration Switch without On board LAN 182 Communication Settings for RS 232C 183 Special Setti...

Page 166: ...memory management ability to update the firmware of the main system Main System Its properties are the complete communication capabilities CAN LAN and RS 232C memory management ability to update the...

Page 167: ...B for the G1315C DAD VVV is the revision number for example 102 is revision 1 02 XXX is the build number of the firmware For instructions on firmware updates refer to section Replacing Firmware in cha...

Page 168: ...ir initial firmware revision For example a G1315C DAD SL cannot be downgraded below firmware revision B 01 02 or to a A xx xx Some modules can be re branded e g G1314C to G1314B to allow operation in...

Page 169: ...page 175 for details Optional Interface Boards The BCD board provides a BCD output for the bottle number of the Agilent 1200 Series autosampler and four external contacts The external contact closure...

Page 170: ...nt modules have one optional board slot that allows to add an interface board to the modules Some modules do not have this interface slot Refer to Interfaces on page 175 for details Table 15 Detailed...

Page 171: ...AN G1369C or G1369 60012 Agilent Technologies Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX recommended for re ordering LAN Communication Interface board G1369A or G1369 60001 Agilent Technologies...

Page 172: ...atures such as start stop common shut down prepare and so on With the appropriate software the RS 232C connector may be used to control the module from a computer through a RS 232C connection This con...

Page 173: ...on the instrument labels provide the following information CCXZZ00000 Format CC Country of manufacturing DE Germany JP Japan CN China X Alphabetic character A Z used by manufacturing ZZ Alpha numeric...

Page 174: ...Electrical Connections Rear view of the module Figure 52 Rear view of detector HZXjg in aZkZg Hadi dg ciZg VXZ WdVgY 6cVad h cVah 6E gZbdiZ GH 8 86C 8dc jgVi dc hl iX EdlZg NOTE The GPIB interface ha...

Page 175: ...C Bin Pump VL 1376A Cap Pump G2226A Nano Pump G5611A Bio inert Quat Pump 2 Yes No Yes 1 Yes G4220A B Bin Pump G4204A Quat Pump 2 No Yes Yes No Yes CAN DC OUT for CAN slaves G1361A Prep Pump 2 Yes No Y...

Page 176: ...G1321B FLD K1321B FLD Clinical Ed G1321C FLD 2 Yes No Yes 2 Yes G1362A RID 2 Yes No Yes 1 Yes G4280A ELSD No No No Yes Yes Yes EXT Contact AUTOZERO Others G1170A Valve Drive 2 No No No No No 1 G1316A...

Page 177: ...No No CAN DC OUT for CAN slaves 1 G4240A CHIP CUBE 2 Yes No Yes No Yes CAN DC OUT for CAN slaves THERMOSTAT for G1330A B NOT USED K1330B 1 Requires a HOST module with on board LAN e g G4212A or G4220A...

Page 178: ...B 06 40 or later or with additional G1369C LAN Card RS 232C Serial The RS 232C connector is used to control the module from a computer through RS 232C connection using the appropriate software This co...

Page 179: ...Output The analog signal output can be distributed to a recording device For details refer to the description of the module s main board Table 18 RS 232C Connection Table Pin Direction Function 1 In...

Page 180: ...critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all c...

Page 181: ...ntrolled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk 5 Not used 6 POWER ON H All modules connected to system ar...

Page 182: ...switch settings depend on configured mode All modules without on board LAN default should be ALL DIPS DOWN best settings Bootp mode for LAN and 19200 baud 8 data bit 1 stop bit with no parity for RS...

Page 183: ...bles for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down and 1 means that the switch is up NOTE The following tables represent the conf...

Page 184: ...data bit with no parity Table 22 Baudrate Settings without on board LAN Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800...

Page 185: ...e in case of firmware loading errors main firmware part If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident mode It is not operable...

Page 186: ...switch settings and power the instrument up again a forced cold start has been completed CAUTION Loss of data Forced cold start erases all methods and data stored in the non volatile memory Exceptions...

Page 187: ...hen housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are virtual elimination of fixing screws bolts or ties reducing the numb...

Page 188: ...n indication that maintenance procedures should be scheduled EMF Counter The detector module provides a EMF counter for the lamp The counter increments with lamp use and can be assigned a maximum limi...

Page 189: ...ment performance indicates maintenance is necessary take note of the values displayed by lamp counters Enter these values or values slightly less than the displayed values as EMF limits and then reset...

Page 190: ...190 Agilent 1260 Infinity VWD User Manual 12 Hardware Information Early Maintenance Feedback EMF...

Page 191: ...Safety Information 192 Batteries Information 195 Radio Interference 196 Sound Emission 197 UV Radiation 198 Solvent Information 199 Declaration of Conformity for HOX2 Filter 201 Agilent Technologies...

Page 192: ...arthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observe...

Page 193: ...zard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do no...

Page 194: ...eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should...

Page 195: ...according to national waste disposal regulations for batteries Replace only with the same or equivalent type recommended by the equipment manufacturer WARNING Lithiumbatteri Eksplosionsfare ved fejla...

Page 196: ...ptimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or...

Page 197: ...on This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position 70 dB Sound...

Page 198: ...is limited to the following TLVs Threshold Limit Values according to the American Conference of Governmental Industrial Hygienists Typically the radiation values are much smaller than these limits Tab...

Page 199: ...ganic solvents for example acetonitrile or methanol 5 Solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of...

Page 200: ...rs should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid i...

Page 201: ...Agilent 1260 Infinity VWD User Manual 201 Appendix 13 Declaration of Conformity for HOX2 Filter Declaration of Conformity for HOX2 Filter...

Page 202: ...inity VWD User Manual 13 Appendix Agilent Technologies on Internet Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at...

Page 203: ...boards ADC 15 C cable analog 154 BCD 159 CAN 161 connecting APG remote 38 connecting CAN 38 connecting LAN 38 connecting the ChemStation 38 connecting the power 38 external contact 163 LAN 161 remote...

Page 204: ...updates 166 135 135 upgade downgrade 135 upgrade downgrade 135 flow cell correction factors 77 exchange 126 high pressure parts 146 micro parts 142 repairing 128 semimicro parts 144 standard parts 139...

Page 205: ...stack configuration 34 overview optical path 11 optical system 11 system overview 11 P packaging damaged 32 parameter setting 78 parameters detector 60 parts for maintenance cuvette holder 148 high p...

Page 206: ...ide 114 intensity of deuterium lamp 104 wavelength calibration 108 test Quick Noise 111 timeout 87 tool kit hplc system 150 troubleshooting available tests vs interface 83 diagnostic signals 80 error...

Page 207: ......

Page 208: ...nt Technologies In This Book This manual covers the Agilent 1260 Infinity Variable Wavelength Detector G1314B C The manual describes the following introcduction and specifications installation using a...

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