10x Genomics Visium CytAssist, User Manual

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CG000542 | Rev A
User Guide | CytAssist Instrument | Troubleshooting
Link to TOC | 10xgenomics.com
Issue
• Upon opening the lid after a successful run, the Visium slide has
popped out of the grooves
on the Visium Slide Stage. This does not
impact assay performance or slide quality.
• Debris is present on the Tissue Slide Stage or Visium Slide stage: see
System Maintenance section to clean the area.
•
Bubbles near tissue on tissue slide
: if bubbles are outside of the
tissue, the risk of affecting assay performance is low. If bubbles are on
top of the tissue, it may result in lack of usable reads in the tissue area
with the bubble.
Best practice: ensure reagent is prepared properly and correctly
dispensed to the wells to avoid introducing bubbles.
Visium Slide Popped out of the Grooves
Debris on the Slide Stage
Debris
Bubble on Tissue
Bubble
Eosin Stained Tissue UMI Counts
Troubleshooting
Some eosin may be washed off during a run,
as shown on the right side of the image.
This does not impact assay performance