10x Genomics Visium CytAssist, User Manual

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CG000542 | Rev A
User Guide | CytAssist Instrument | Tips & Best Practices
Link to TOC | 10xgenomics.com
f.
If necessary, rotate the slide 180° as shown to better place off-center tissues
closer to the center line. Slides should not cross/overlap the center line.
Refer to the Visium CytAssist Training Kit User Guide (CG000549) for further guidance.
Load Tissue Slides onto the Tissue Slide Stage
a.
Press down on the wide end of clip to lift and pivot the narrow end of
the clip. Pivot the clips to their outermost position.
Examples of Good Slide Placement on the Tissue Slide Stage
right side of the stage
rotated & still on the
right side of the stage
OR
F
F
Slide “F” shown with a 6.5 mm section:
left side of the stage
OR
rotated & still on the
left side of the stage
G
G
Slide “G” shown with a 11 mm section:
Tissue Slide
Placement on
Tissue Slide Stage
contd.
Overlay of a 11 mm fiducial frame
and the alignment guides
Note that the outside of the
alignment guides mark the
boundary of the fiducial frame on
a Visium CytAssist Slide Capture
Area.
When placing a tissue slide on
the Tissue Slide Stage, tissue may
come up to the edge of the 11 mm
alignment guides. boundary
Press down on the wide
end of the clip