10x Genomics Visium CytAssist, User Manual

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CG000542 | Rev A
User Guide | CytAssist Instrument | Tips & Best Practices
Link to TOC | 10xgenomics.com
Check Slide Dimensions
While slides are specified as being 25 mm x 75 mm, manufacturing
tolerances may lead to dimensions that are too small or large to be
compatible with Visium CytAssist. Tissue slide dimensions must be
within 24.8 mm - 25.3 mm in width and 74.4 mm - 76.2 mm in length to
fit the Visium CytAssist Tissue Slide Cassette.
•
Minimum slide dimensions: 24.8 x 74.4 mm
•
Maximum slide dimensions: 25.3 x 76.2 mm
Check Tissue Placement on Slide
Use this section to determine if the tissue section is located in an area that results in
successful analyte transfer and imaging. The Visium CytAssist Quick Reference Cards –
for Accessory Kit Instruction (CG000548), located within the Instrument Accessory Kit
(laminated document with concise instructions).
a.
Ensure that the tissue region of interest will fit within the Capture
Area of a Visium CytAssist Spatial Gene Expression Slide. Overlay the
slide, centering the tissue on either the 6.5 mm or 11 mm square.
b.
If the slide has no frosted areas, overlay on this diagram. The tissue
should lie within the green allowable area:
• 15 mm from the short sides
• 5 mm from the long sides
Ensure that the slide
surface with the tissue
is facing the user.
11 mm
Capture Area
6.5 mm
Capture Area
15 mm
15 mm
5 mm
Tissue outside this region cannot
be analyzed
5 mm
If printing this page:
Select “actual size” or
“100%” to print to scale.
The slide diagram should
measure 25 mm x 75 mm.
Icons
Tips & Best Practices section
includes additional guidance
Signifies critical step
requiring accurate execution
Troubleshooting section
includes additional guidance
Check Tissue
Placement on Slide