Zeiss LSM 700, Быстрое руководство

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01/2013
23
Scanning a Z-Stack
x
Select Z-Stack in the main
tools area.
x
Open the Z-Stack tool in the Left Tool Area .
x
Click on the button in the Action
Button area.
A continuous XY-scan of the set focus position
will be performed.
x
Use the focus drive of the microscope to focus
on the upper position of the specimen area
where the Z-Stack is to start.
x
Click on the Set First button to set the upper
position of the Z-Stack.
x
Then focus on the lower specimen area where
the recording of the Z-Stack is to end.
x
Click on the Set Last button to set this lower
position.
x
Click on the button to set number of slices to match the optimal Z-
interval for the given stack size, objective lens, and the pinhole diameter.
x
Click on the Start Experiment button to start the recording of the Z-Stack.
When a multi-dimensional acquisition tool is not selected, the respective tool and its set parameters
are not included in the multidimensional image acquisition. If no multidimensional tool is activated,
the Start Experiment button is grayed out and only single images can
be scanned.
Fig. 26 Z Stack tool