Zeiss LSM 700, Быстрое руководство

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20
01/2013
Setting scan averaging
Averaging improves the image by increasing the signal-to-noise ratio. Averaging scans can be carried out
line-by-line or frame-by-frame. Frame averaging helps to reduce photo-bleaching, but does not give quite
as smooth of an image.
x
For averaging, select the Line or Frame mode in the Acquisition Mode tool.
x
Select the number of lines or frames to average.
Adjusting pinhole
x
Select the Channels tool in the Left Tool Area .
x
Set the Pinhole size to 1 AU (Airy unit) for best compromise between depth discrimination and
detection efficiency.
Pinhole adjustment changes the Optical Slice thickness . When collecting multi-channel images, adjust
the pinholes so, that each channel has the same Optical Slice thickness . This is important for
colocalization studies.
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Fig. 21 Channels tool