Zeiss LSM 700, Быстрое руководство

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01/2013
11
Setting up the microscope and storing
settings
Click on the Locate tab for direct observation;
press the Oculars Online button for your actions
to take effect immediately. Then open the Ocular
tool to configure the components of your
microscope like filters, shutters or objectives
(Fig. 11).
Selecting an objective
x
Open the graphical pop-up menu by clicking on
the
Objective symbol and select the objective
lens for your experiment (Fig. 11).
The chosen objective lens will automatically
move into the beam path.
Focusing the microscope for transmitted light
x
Open the graphical pop-up menu by clicking on
the
Transmitted Light icon (Fig. 12).
x
Click on the On button. Set the intensity of the
Halogen lamp using the slider.
x
Clicking outside the pop-up control closes it.
x
Place specimen on microscope stage. The cover
slip must be facing the objective lens.
Remember the immersion medium if the
objective chosen requires it!
x
Use the focusing drive of the microscope to
focus the object plane.
x
Select specimen detail by moving the stage in X
and Y using the XY stage fine motion control.
Fig. 11 Microscope Control window, e.g.:
Axio Observer