Image Acquisition
If you activate the
Apply to all T
check box the settings in all the acquired
instants of exposure are applied to the relevant fluorescence channel.
Note:
•
You can get the best display of colored objects, if the gamma setting is in
the range of approx. 0,45.
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Activate the
Enhance Color
check box on the
General
property page to
improve the color reproduction quality (this slows down the live image rate
slightly as additional calculations are required).
The standard setting installed for 3200 K (
3200 K
button on the
Adjust
property page) often offers the camera the most natural reproduction of the
image situation on the microscope. Here the image background is reproduced
as it appears on the microscope. This setting is also useful in acquisition
situations where no neutral-tint area is available to carry out a balance
manually, e.g.: fluorescence.
If the image background in the acquired image is to be displayed with a
neutral tint (gray), a manual white balance is required. Click on the
White
Balance
button; then click on an area in the image that has a neutral tint and
is not overexposed.
If the lamp voltage selected on the microscope is too low, the color of the
image background may have been shifted a long way into the red. If a white
balance is carried out in this event, the result may be visible deviations in the
display of the color of the current sample. Correct this situation by increasing
the lamp voltage to achieve a more neutral background, and insert gray filters
into the microscope’s illumination beam path to adjust the intensity.
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The output medium has a significant influence on color perception!
Use a familiar specimen to adjust the brightness and contrast rendering. If
possible also set the color temperature of the monitor to approx. 5000 K or
6500 K.
AxioCam MR Instal Reference
73
