Image Acquisition
Notes:
•
The reference images generated are saved on a user-specific basis. All
users who have their own login must therefore generate their own set of
reference images.
•
Automatic shading correction only takes into account the changing of the
objective. Changes to the settings of other optical components, such as
an Optovar, the camera adapter or the condenser, are not taken into
account and will make it necessary to repeat the generation of the
reference images.
•
If several cameras are operated on a system (e.g. an AxioCam MRm and
an AxioCam MRc5), the reference images for each camera will managed
separately.
•
In the
Multidimensional Acquisition
function, a function for
fluorescence-channel-specific shading correction is offered under
Extended parameters
on the
C (Channel)
property page.
•
Activating shading correction can reduce the maximum possible speed of
the live image.
Applying object-specific shading correction
Activate the
Automatic
check box on the camera property page
General
.
Note:
•
Make sure that the shading reference images generated are still having
the desired effect if the images were generated some time ago. They will
need to be generated again in any case if the optical settings of the
microscope have been changed. This applies to the following settings in
particular:
•
Lamp adjustment
•
Köhler setting
•
Optovar
•
Camera adapter
•
Camera port
AxioCam MR Instal Reference
59