Image Acquisition
Applying shading correction manually
With non-motorized and non-encoded microscopes the automatic shading
correction can not be activated. But it is possible to carry out shading
correction using a previously generated standard reference image. This image
is applied irrespective of the objective set.
To generate this file, follow the procedure described above. However, this
function can only be carried out for one objective. As a compromise for all
available objectives, you can perform this using an objective with a medium
magnification. For special requirements you will need to carry out shading
correction again, specifically for the objective you are using, prior to the
acquisition of an image.
Shading correction via image processing
For advanced users it is also possible to carry out shading correction via an
image processing function. On the
Processing
menu
⇒
Adjust
⇒
Shading
Correction
you will find a function that allows you to perform targeted
subsequent processing. This function can be used in a script to allow you, for
example, to generate and apply an image with an assigned shading correction
for each objective.
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With this function, it is possible for images to receive further processing using
a sharpness algorithm immediately after acquisition by the camera without an
image-processing function having to be started manually. The impression of
sharpness in the image is increased and it is therefore possible for extremely
attractive images that are rich in detail to be displayed on the monitor without
delay.
The technique applied is employed by many image-processing programs and
is known as
Unsharp Masking
. Here, the contrasts in small structures and
edges are increased in a targeted way, leading to an enhanced impression of
sharpness.
60
M 60-3-0014 e / printed 12.2008
