21
c)
If you have selected multiple contrast methods that require distinct
illumination methods (e.g. Bright Field and Fluorescence), you must use the
Wait
block.
To set a
Wait
block, just click in the
Wait
button.
As it is strictly required to operate the diaphragm slide between transitions of
transmitted and incident light (e.g. from bright field to fluorescence GFP), the
Wait
block
will make a pause during the experiment.
At the pause, you should set the diaphragm slide in agreement with the next
contrast method.
To switch the order among blocks, just click in a block and move it into the
desired position of the timeline of events.
10.
Box the
1 separate image document / acquisition block
option to create an
image for each block of the timeline of events