20
9.
In the
Acquisition
Block section, click in the
Add New
button.
a)
Add as many blocks as contrast methods/filter sets you would like to
acquire. For instance, to acquire images of Bright Field, GFP, mRFP and YFP, four blocks
must be added. This approach will generate one image for each contrast method and
filter set.
Later, you will be able to combine two images at a time into one using ZEN 2.1
(See ZEN 2.1 (blue edition) Guidelines -> Channel Combine or Image Overlay)
b)
Alternatively, you can add only one block. This approach will generate a
multichannel image of all selected filter sets. Combine only filter sets into a single block
though, as it is required one block for each single contrast method.
In the end, you can extract individual fluorescence images of a multichannel
image (See ZEN 2.1 (blue edition) Guidelines -> Extracting Single Fluorescence Images of