Zeiss Axio Scope.A1, Руководство по эксплуатации

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OPERATION
Carl Zeiss Illumination and Contrasting Method Axio Scope.A1
94
M60-2-0007 e 05/08
•
Switch on the lamp HBO 100 or HBO 50
(
691H691H
Fig. 4-16/ 1 ) and let it warm up to operational
temperature for about 15 min.
•
On the reflector turret/slider (
692H692H
Fig. 4-16/ 6 ),
choose the reflector module FL P&C with the
desired fluorescence filter combination
(depending on the excitation mode) and switch
it on.
•
Now unblock the light path in the reflected
light illuminator with the filter slider reflected
light (
693H693H
Fig. 4-16/ 2 ).
•
If necessary, adjust FL attenuator to 100 %
transmission in order to facilitate locating
fluorescence signals. Reduce the transmission
later on in order to preserve the sample.
•
Take out an eyepiece from the tube and adjust
the aperture diaphragm at sight. Open the
compartment for the aperture diaphragm for
P&C (
694H694H
Fig. 4-16/ 3 ) enough to see the whole
objective exit pupil.
•
Replace the eyepiece in the tube and close the
field diaphragm (
695H695H
Fig. 4-16/ 4 ) enough to make it
visible in the field of view.
•
Using both centering screws (
696H696H
Fig. 4-16/ 5 ), center the field diaphragm on the edge of the field of view.
•
Open the field diaphragm enough to make it just disappear behind the edge of the field of view, or, if
you are using a sample which might bleach out, reduce the field diaphragm into the field of view.
•
Focus the sample again and optimize the collector position of the HBO 100. Adjust the collector so
that the reflector module of the short-wave excitation illuminates the field of view consistently. A
correction of the collector position is not necessary in modules with longer-wave excitation.
Fig. 4-16 Components for reflected light
fluorescence