Zeiss ApoTome.2, Руководство по эксплуатации

Страница: 11 / 34

INSTRUMENT DESCRIPTION
ApoTome.2 Product name and intended use Carl Zeiss
05/2012 423667-7144-001 11
2 INSTRUMENT DESCRIPTION
2.1 Product name and intended use
Manufacturer's product name: ApoTome.2 for Axio Imager (423667-9100-000)
ApoTome.2 for Axio Observer and Axiovert 200
(423667-9000-000)
ApoTome.2 for Axio Zoom.V16 (423667-9200-000)
The ApoTome.2 allows depth-discriminated images (= optical sections) of fluorescence specimens to be
produced. Compared to the conventional reflected light fluorescence methods, these optical sections
feature increased contrast and enhanced optical resolution in axial direction. Furthermore, optical
sections through the specimen are the prerequisite for the three-dimensional reconstruction of structures.
2.2 Instrument description and main features
The ApoTome.2 hardware consists of two components:
1. slider with transmission grids changer
2. control box
The slider is directly connected to the control box via a cable. The control box is connected to the PC by a
USB cable or, optionally, it can be connected directly to the microscope by a CAN BUS cable. In this case,
the ApoTome.2 directly communicates with the PC via the electronic system of the microscope.
If an Axiovert 200 is used, the control box can only be connected to the PC by a USB cable. A
connection via CAN BUS is not possible!
Major features of the
ApoTome.2
include:
−
ApoTome.2 slider for the plane of the luminous-field diaphragm in the reflected light path
−
ApoTome.2 slider with two click-stop positions:
Click-stop position 1:
An open passage is provided in the reflected light path. Thus, normal fluorescence observations can be
carried out in the wide field, e.g. to find interesting structures and to position the specimens.
Click-stop position 2:
In this position, a glass plate with evaporated grid structure is in the light path. The grid structure is
laterally moved in the specimen plane by means of a scanner mechanism. By capturing three (or more)
images at different grid positions and by subsequent calculation, it is possible to produce an optical
section through the specimen. Three different grid frequencies selected via software are available.
They allow the user to obtain different section thicknesses and to use different objectives.