X-Cite Exacte, Руководство пользователя

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X-Cite
®
exacte User Guide
035-00283 rev 3
7.2 X-Cite
®
exacte Calibration using the XR2100 (or XR2000) & Light Guide
7.2.1 Set up the XR2100 to obtain a power reading through the light guide input port.
Refer to section 7.1 or user guide 035-00390R.
7.2.2 Attach the Stereo Connection Cable to the port on the right side of the XR2100
(under flap in rubber boot), and the port on the right side of the X-Cite
®
exacte .
7.2.3 Press and release the X-Cite
®
exacte CAL button on the XR2100.
7.2.4 The X-Cite
®
exacte will display CAL, and the XR2100 will display a moving dash
(
– ) while the calibration sequence is running.
7.2.5 When the calibration is complete, the CAL LED on the X-Cite
®
exacte will light
up, and the X-Cite
®
exacte will display intensity settings in the format XX.XX
(in watts).
7.2.6 For versions 2.0 and up: X.XXX or XXX.X for milliwatts and uXXX for microwatts.
WARNING: Do not remove the light guide from the Power Meter until calibration
sequence is complete. While calibrating, the shutter will
automatically open and close under the control of the power meter.
7.3 Calibrating X-Cite
®
exacte with the Objective Plane Power Sensor,
XP750
7.3.1 For convenience, the basic instructions for using the Objective Plane Power
Sensor with the X-Cite
®
exacte are provided here. For full instructions and safety
information, refer to the complete Optical Power Measurement S
ystem User’s
Guide (035-00390R).
7.3.2 Leave the X-Cite
®
exacte light guide connected to your microscope as you would
during normal operation. Keep the X-Cite
®
exacte positioned as you would
during normal operation.
7.3.3 Place the XP750 onto your microscope as described in user guide 035-00390R,
and connect it to an XR2100.
7.3.4 Attach the Stereo Connection Cable to the port on the right side of the XR2100
(under flap in rubber boot), and the port on the right side of the X-Cite
®
exacte .
7.3.5 IMPORTANT: The XP750 will calibrate X-Cite
®
exacte using data from only one
wavelength setting. It is important to select the wavelength most applicable to
your application prior to calibrating the X-Cite
®
exacte using the system. The
ideal wavelength setting is dependent on your application and typically
corresponds to the excitation maxima (peak) of the fluorophore. Please refer to