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Nanoliter 2000

World Precision Instruments 

19

* Electrodes, batteries and other consumable parts are warranted for 30 days only from the date on which the 
customer receives these items.

WARRANTY

WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, includ-
ing its components and parts, shall be free from defects in material and workmanship for a period of 
30 days* from the date of receipt. WPI’s obligation under this warranty shall be limited to repair or 
replacement, at WPI’s option, of the equipment or defective components or parts upon receipt thereof 
f.o.b. WPI, Sarasota, Florida U.S.A. Return of a repaired instrument shall be f.o.b. Sarasota.

The above warranty is contingent upon normal usage and does not cover products which have been 
modified without WPI’s approval or which have been subjected to unusual physical or electrical stress 
or on which the original identification marks have been removed or altered. The above warranty will 
not apply if adjustment, repair or parts replacement is required because of accident, neglect, misuse, 
failure of electric power, air conditioning, humidity control, or causes other than normal and ordinary 
usage.

To the extent that any of its equipment is furnished by a manufacturer other than WPI, the foregoing 
warranty shall be applicable only to the extent of the warranty furnished by such other manufacturer. 
This warranty will not apply to appearance terms, such as knobs, handles, dials or the like.

WPI makes no warranty of any kind, express or implied or statutory, including without limitation any 
warranties of merchantability and/or fitness for a particular purpose. WPI shall not be liable for any 
damages, whether direct, indirect, special or consequential arising from a failure of this product to 
operate in the manner desired by the user. WPI shall not be liable for any damage to data or property 
that may be caused directly or indirectly by use of this product.

Claims and Returns

Inspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be noted 
on the delivery receipt before signing. Concealed loss or damage should be reported at once to the 
carrier and an inspection requested. All claims for shortage or damage must be made within ten 
(10) days after receipt of shipment. Claims for lost shipments must be made within thirty (30) days 
of receipt of invoice or other notification of shipment. Please save damaged or pilfered cartons until 
claim is settled. In some instances, photographic documentation may be required. Some items are 
time-sensitive; WPI assumes no extended warranty or any liability for use beyond the date specified 
on the container
Do not return any goods to us without obtaining prior approval and instructions from our Returns 
Department. Goods returned (unauthorized) by collect freight may be refused. Goods accepted for 
restocking will be exchanged or credited to your WPI account. Goods returned which were ordered 
by customers in error are subject to a 25% restocking charge. Equipment which was built as a special 
order cannot be returned.

Repairs

Contact our Customer Service Department for assistance in the repair of apparatus. Do not return 
goods until instructions have been received. Returned items must be securely packed to prevent fur-
ther damage in transit. The Customer is responsible for paying shipping expenses, including adequate 
insurance on all items returned for repairs. Identification of the item(s) by model number, name, as 
well as complete description of the difficulties experienced should be written on the repair purchase 
order and on a tag attached to the item.

Содержание Nanoliter 2000

Страница 1: ...Nanoliter 2000 Microprocessor controlled nanoliter injection World Precision Instruments INSTRUCTION MANUAL Serial No _____________________ 092512 www wpiinc com ...

Страница 2: ......

Страница 3: ...troller Buttons 5 Plunger in HOME Position 6 OPERATING INSTRUCTIONS 7 Micropipette Pulling 7 Securing the Micropipette to the Injector 7 Micropipette Filling Techniques 8 Back Filling 8 Front Filling 9 Typical Technique 9 Injecting 9 Setting the Aliquot Volume and Injection Speed 10 MAINTENANCE 11 Replacing O Rings 11 Replacing the Wire Plunger 11 Cleaning Recommendations 12 OPTIONAL ACCESSORIES 1...

Страница 4: ...iv World Precision Instruments ...

Страница 5: ...bol indicates a WARNING Warnings alert you to actions that can cause personal injury or pose a physical threat Please read these carefully NOTES and TIPS contain helpful information 40m m Reversing mount 180º will reduce manipulator to tip distance by 10cm Mounting Rod 8 2Ø x 50mm 1 3 5 c m 2 5 7 5 c m Total volume in pipette 1 3 4 9 17 4µL Universal Adapter for Nanoliter Injector Fig 1 Nanoliter ...

Страница 6: ...archers report using 30µm tips with no increase in mortality Injections of 25nL or less generally require smaller tips normally 10µm A broken tip is preferable for oocyte injection Sharp polished tips tend to deflect An excellent reference for all procedures involving the Xenopus oocyte is Kay B and H Peng Xenopus Iaevis Practical Uses in Cell and Molecular Biology Methods in Cell Biology Vol 36 A...

Страница 7: ...eturns on page 19 of this manual Please contact WPI Customer Service if any parts are missing at 941 371 1003 or customerservice wpiinc com Returns Do not return any goods to WPI without obtaining prior approval RMA required and instructions from WPI s Returns Department Goods returned unauthorized by collect freight may be refused If a return shipment is necessary use the original container if po...

Страница 8: ...able The injector head can be mounted in a micromanipulator WPI s M3301 model is quite suitable and frequently used The 500778 Universal Adapter for the Nanoliter Injector shaft diameter of 8 2mm is used for mounting the Nanoliter Injector to a micromanipulator The Nanoliter 2000 is comprised of a controller Fig 3 and an injector handle Fig 4 Fig 3 shows the right side of the controller where the ...

Страница 9: ...from end of collet The empty speed is approximately 92nL s Fast Fill and Fast Empty DIP switch 5 has no effect on the fast empty mode To empty rapidly hold down the Empty button and touch the Fill button one time The empty speed increases to 230nL s The plunger maintains the faster speed until the Empty button is released The fill mode also has a fast speed Hold down the Fill button and touch the ...

Страница 10: ...f the wire plunger should be slightly recessed from the end of the collet This prevents accidental damage to the plunger when it is not in use To reset the plunger to this position push the Fill button to retract or the Empty button to extend the plunger and simultaneously hold the Inject button When in the home position release both buttons to reset the unit ...

Страница 11: ...pically backfilled with oil before attachment to the injector Silicone or mineral oil is frequently used Backfilling is facilitated by using the flexible MicroFil non metallic needle and a syringe NOTE Nanoliter 2000 will not operate properly without backfilling the micropipette Securing the Micropipette to the Injector The injector is supplied with the standard collet O ring configuration Standar...

Страница 12: ... the non pulled end which has the relatively large opening that allows for rapid bulk filling Front side the pulled end of the micropipette the sharp side The filling method of choice is usually dictated by experimental requirements and is chosen at your discretion Back Filling The term back filling refers to the process of filling the pipette from the large non pulled end Back filling offers a gr...

Страница 13: ...tte with a small volume for microinjection 1 Backfill the micropipette with mineral oil 2 Use the Nanoliter 2000 controller to eject the plunger from the home position This is necessary in order to allow the plunger to move backwards to create a vacuum for front filling 3 Fig 5 and Fig 6 show the assembly of the micropipette to the Nanoliter 2000 4 Once the micropipette is installed submerge the m...

Страница 14: ... side of the control box Table 1 lists the DIP switch settings to use for DIP switches 1 4 to select the various aliquot volumes U up D down DIP switch 5 determines the fast injection and fill rates Up Fast 46nL s Down slow 23nL s TIP To set the DIP switches use a ball point pen or a jeweler s flat head screw driver NOTE DIP switch settings for the Nanoliter 2000 are completely different from the ...

Страница 15: ...your other hand This exposes a brass fitting with two Allen screws on opposite sides of the brass fitting and the clear stop CAUTION Do NOT disassemble the Motor Housing Doing so may damage the plunger drive assembly 4 Using the 0 05 hex wrench provided with the replacement plunger loosen the two set screws NOTE The 0 035 hex wrench is for use with the Nanoliter 2010 5 Remove the plunger 6 Insert ...

Страница 16: ...clave the Nanoliter 2000 The control box may be cleaned by wiping it with a clean damp cloth OPTIONAL ACCESSORIES Table 2 Accessories Part Number Description 4878 Replacement 3 5 in glass capillaries 300 4879 Replacement 7 in glass capillaries 300 300521 Spare Parts Kit includes MicroFil MF34G displacement plunger five O ring sets tools 13142 Footswitch for Nanoliter 2000 15867 Footswitch for Micr...

Страница 17: ...ank Plunger doesn t retract completely Home position is set incorrectly See Plunger in HOME Position on page 6 for instruction on resetting the home position NOTE If you have a problem issue with that falls outside the definitions of this troubleshooting section contact the WPI Technical Support team at 941 371 1003 or technicalsupport wpiinc com SPECIFICATIONS The Nanoliter 2000 conforms to the f...

Страница 18: ...CTION MANUAL Table 3 Micro4 Volume Setting Table Type Syringe Volume Scale Length ID mm nL step REV K Max Rate nL s Max Rate Microstep Mode nL s A 0 5µL 54 1mm 0 1085 0 0294 20 1 B 1 0µL 54 1mm 0 1534 0 0587 40 2 C 5µL 54 1mm 0 343 0 2934 202 14 D 10µL 54 1mm 0 485 0 5868 451 29 E 25µL 60mm 0 73 1 329 1022 66 F 50µL 60mm 1 03 2 646 2035 132 G 100µL 60mm 1 46 5 315 4088 265 H 250µL 60mm 2 3 13 191 ...

Страница 19: ...8 9 One of these two volumes should be used to insure a proper injection By default if you leave the value on the Micro4 at 100nL 98 9nL is injected Difficulty can arise when the volume value is half or more of the next 2 3nL step For example setting the Micro4 for an injection of 10nL results in a theoretical injection volume of 9 2nL produced by 4 whole steps of the injector Five whole steps res...

Страница 20: ...16 World Precision Instruments ...

Страница 21: ...witch 5 9 10 DIP switches 9 DIP switch settings 10 E Empty 5 F Fast Empty 5 Fast Fill 5 filament 7 Fill 5 H home position 6 7 11 I Inject 5 injection speeds 10 L leakage 11 M Micro4 Volume 14 MicroFil 2 7 12 micromanipulator 4 micropipette 9 O oocyte 2 P parts list 2 plunger 2 11 13 R returns 3 S seal 13 softening point 7 specifications 13 T TIP10XV119 12 troubleshooting 13 U unpacking 3 W wire pl...

Страница 22: ...18 World Precision Instruments DECLARATION OF CONFORMITY ...

Страница 23: ...rect special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or indirectly by use of this product Claims and Returns Inspect all shipments upon receipt Missing cartons or obvious damage to cartons should be noted on the delivery receipt before signing Conceale...

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