WPI Nanoliter 2000, Инструкция по эксплуатации

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14 World Precision Instruments
APPENDIX: NANOLITER 2000/MICRO4 VOLUME
SETTINGS
When using the Micro4 to control injections with the Nanoliter 2000 , take
care when entering the injection volume. The Nanoliter 2000 injector’s
volume per step is based on the movement of the plunger wire inside a
pulled glass pipette. This plunger moves 0.0005" (12.7µm) for each step of
the motor. The volume of 2.3nL per step is based on the inside diameter of
a 0.5mm pipette and the 12.7µm movement of the plunger wire. As shown
in the table below, use Type K when programming the
Micro4 for use with
the Nanoliter 2000 .
NOTE: FOR FULL OPERATING INSTRUCTIONS FOR THE MICRO4 AS IT
RELATES TO THE NANOLITER 2000 OPERATION, REFER TO THE UMP3
INSTRUCTION MANUAL.
Table 3: Micro4 Volume Setting Table
Type
Syringe
Volume
Scale
Length
ID (mm)
nL / step REV
K
Max.
Rate
nL/s
Max. Rate
Microstep
Mode nL/s
A 0.5µL 54.1mm 0.1085 0.0294 20 1
B 1.0µL 54.1mm 0.1534 0.0587 40 2
C 5µL 54.1mm 0.343 0.2934 202 14
D 10µL 54.1mm 0.485 0.5868 451 29
E 25µL 60mm 0.73 1.329 1022 66
F 50µL 60mm 1.03 2.646 2035 132
G 100µL 60mm 1.46 5.315 4088 265
H 250µL 60mm 2.3 13.191 9999 659
I 500µL 60mm 3.26 26.501 9999 1325
J 1000µL 4.61 52.995 9999 2649
K
Nanoliter
2000
0.48
plunger
in 0.50
glass
2.3 nL /step
(0.0005” step)
884 115
L 10µL 60mm 0.4607 0.5293 407 29
M N
O P
User
Defined
See page 14
custom
rate
*
** ILS005 0.4856
0.5880
compensates
for length as
TYPE M
*The rate maximum is calculated internally and is determined by the nL/
step value times 10000 and divided by 13. The microstepping maximum
rate is nL/step*10000/200.
A203XVY (original Nanoliter ) uses type M, N, O or P with a value=1.15nL/step.