WPI Nanoliter 2000, Инструкция по эксплуатации

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2 World Precision Instruments
INTRODUCTION
The Nanoliter 2000 microinjection system provides you with the capability
to inject liquids in discrete aliquots ranging from 2.3nL to 69nL. The
Nanoliter Injector utilizes a stainless steel plunger to create a mechanical
displacement of fluid within a micropipette, thus pushing the contents out
through the pipette tip.
General Notes
• The wire plungers on all injectors have a point on one end. This wire
can be reversed if a blunt end is desired. The point should allow bubbles
in the oil to escape when backfilling tips.
• The preferred tip opening size for Xenopus injection is about 20µm.
Some researchers report using 30µm tips with no increase in mortality.
• Injections of 25nL or less generally require smaller tips, normally 10µm.
• A broken tip is preferable for oocyte injection. Sharp polished tips tend
to deflect.
• An excellent reference for all procedures involving the Xenopus oocyte
is Kay, B. and H. Peng, “Xenopus Iaevis: Practical Uses in Cell and
Molecular Biology.” Methods in Cell Biology, Vol. 36, Academic Press.
Parts List
After unpacking, verify that there is no visible damage to the instrument.
Verify that all items are included:
(1) Injector head
(1) Control unit
(1) Power supply
(1) 500778 Universal Adapter for Nanoliter Injector
(2) Capillary glass vials
(1) 3.5” capillaries, 100 pieces
(2) 7.0” capillaries, 100 pieces
(1) Replacement O-ring kit, standard
(2) Allen wrenches for replacing wire plunger (0.035” for Nanoliter 2010,
0.050” for Nanoliter 2000)