WPI Nanoliter 2000, Инструкция по эксплуатации

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Nanoliter 2000
World Precision Instruments 7
OPERATING INSTRUCTIONS
The precise operation of the Nanoliter 2000 depends greatly on the use of
tips prepared from the glass provided. Micropipettes pulled from capillaries
with other dimensions may not work.
CAUTION : Never attempt to use micropipettes pulled from glass
containing a filament. Damage to the wire plunger will result and
injection volumes will not be accurate.
Micropipette Pulling
Ideally, the tip size should be pulled to 10-30µm. The capillary glass
provided has a softening point of 780°C. Many researchers pull the tips
smaller than required and then (using forceps) break them off at the desired
size. The sharp broken edge works well to pierce the cell membrane.
Once the tips are pulled, they are typically “backfilled” with oil before
attachment to the injector. Silicone or mineral oil is frequently used.
Backfilling is facilitated by using the flexible MicroFil™ non-metallic needle
and a syringe.
NOTE : Nanoliter 2000 will not operate properly without backfilling the
micropipette.
Securing the Micropipette to the Injector
The injector is supplied with the standard collet/O-ring configuration.
Standard Collet/O-ring Configuration
1. Once the micropipette is backfilled, loosen the collet (
Fig. 5
). The
pointed wire plunger should be positioned so you can just see the tip
flush with the end of the collet (slightly recessed is also acceptable). This
is referred to as the “home” position.
2. Push the micropipette onto the wire plunger. As you push the tip on,
feel it go through the large O-ring and seat in the white spacer. See Fig.
5 for the proper configuration of the O-rings and the white spacer. It is
absolutely essential that these components are properly configured.