WPI Nanoliter 2000, Инструкция по эксплуатации

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8 World Precision Instruments
Sealing “O” ring (small hole)
Aluminum Barrel
Plunger
Plastic Sleeve (spacer)
placed between “O” rings
with indentation toward glass
“O” ring with large hole
Collet (K3)
Glass
Fig. 5
–Standard configuration for installing the micropipette
3. Once positioned, tighten the collet securely. Give the micropipette a pull
to confirm it is securely mounted.
NOTE
: The white spacer has one flat side and one side with a recess
machined around the hole. This recess receives the back end of the
pipette and protects the plunger sealing O-ring from damage.
Micropipette Filling Techniques
There are various methods for loading liquid samples into micropipettes for
microinjection. A pulled micropipette has a back side and a front side.
• Back side–the non-pulled end, which has the relatively large opening
that allows for rapid bulk filling
• Front side–the pulled end of the micropipette, the sharp side
The filling method of choice is usually dictated by experimental
requirements and is chosen at your discretion.
Back Filling
The term “back filling” refers to the process of filling the pipette from
the large, non-pulled end. Back filling offers a great advantage when
performing a bulk fill, because the large opening allows you to use a
syringe and MicroFil flexible needles. (See “Optional Accessories” on page
12.) The bulk filling process occurs rapidly with little mess.
1. Attach a MicroFil needle to a syringe, immerse the tip of the needle into
the filling liquid and use the syringe to pull the fluid into the MicroFil
needle.
2. Place the MicroFil needle into the micropipette from the back side so
that the needle’s tip is pushed all the way into the front part of the
pipette near the pulled end.