Sterilization Cycle
Information You Need to Know Before Starting a Cycle
• The Sterilization Cycle requires approximately 12 hours -
heat-up (2-4 hours), sterilize (2 hours), and cool down (6-8 hours).
Additional time is needed to verify the calibration of temperature and CO
2
after the cycle is
complete.
• During the Sterilization Cycle, the incubator updates the temperature to the analog output
board and the 1535, however CO
2
will be fixed at setpoint and RH will be fixed at RH
Low Limit plus 1%.
• A HEPA filter replacement kit (see the spare parts list) should be on hand prior to
initiating the Sterilization Cycle.
Information About the Cycle
• Pre-cleaning may be required. In order to avoid odors, stains on the interior, baked-on
material, etc., wipe off all visible signs of spills.
• Odor may occur during the Sterilization Cycle and is considered normal.
• The Steri Cycle is not intended to sterilize other items; instruments, etc., from the lab.
• During the cycle, the unit chamber becomes hot enough to melt samples, instruments,
dishes, etc., left inside the unit. The items listed below also need to be removed.
1) HEPA filter
2) Air sample filter
3) Access port filter assembly
4) Water in the humidity pan
5) Temp/RH recorder probe, if applicable
6) IR sensor, if applicable
• During the Sterilization Cycle, discoloration
of some materials may occur. For example,
stainless steel turns a straw color after a period
of exposure to high temperatures. This is
normal.
Air Sample
Filter
and tubing
Access
Port Filter
and stopper
assembly
HEPA
Filter
Humidity Pan
Air Jacketed CO2 Incubator
5-5
VWR International
Section 5
Routine Maintenance
