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Plantar Test

(Hargreaves Apparatus)

General

Determination of acute nociceptive thermal threshold in laboratory animals has primarily relied upon the tail flick and

hot plate methods.
Although both methods are used frequently in pharmacological studies, they are not without limitation. In addition,

neither method has been extended to investigating behavioural responses to hyperalgesia.
The Plantar Test represents a remarkable advance in methodology, as it combines the best features of all other methods

of measuring pain sensitivity. Unique to the Plantar Test,

the animal is unrestrained and unhandled during experi-

ments.

PAIN and INFLAMMATION

For Rats

Cat. No. 37370

AUTOMATIC

MEASUREMENT OF THE

ANIMAL RESPONSE

Main Features

Automatic detection of paw with-

drawal (no visual score needed!)

I.R. intensity adjustable in the

interval 01-99 (in one digit steps)

Software included

Modular animal enclosure, from 3 to 12 spaces,

conveniently designed to restrain mice or rats

Optional 37300 Radiometer for calibration

Data portability via the included memory key

NEW: orofacial stimulation by optional holders

For Mice

Ugo Basile: more than 10,000 citations

Содержание 37370

Страница 1: ...UGO BASILE S R L sales ugobasile com service ugobasile com www ugobasile com instruction manual Plantar Test Hargreaves Apparatus Cat No 37370...

Страница 2: ...D TO ENSURE SAFE OPERATION AND TO RETAIN THE INSTRUMENT IN SAFE CONDITIONS SERVICE AND ADJUSTMENTS SHOULD BE CARRIED OUT BY QUALIFIED PERSON NEL AUTHORIZED BY UGO BASILE ORGANIZATION ANY ADJUSTMENT MA...

Страница 3: ...t features of all other methods of measuring pain sensitivity Unique to the Plantar Test the animal is unrestrained and unhandled during experi ments PAIN and INFLAMMATION For Rats Cat No 37370 AUTOMA...

Страница 4: ...USIBILI 2AF E AU 041 USB pen drive 37370 302 1 INSTRUCTION MANUAL MANUALE DI ISTRUZIONE 52050 10 CUB SOFTWARE SOFTWARE CUB 52010 323 1 USB CABLE CAVO USB OPTIONAL 37000 145 1 PANEL MOUNT PRINTER STAMP...

Страница 5: ...INSTALLATION 6 4 1 UNPACKING PRELIMINARY CHECK 6 4 2 NOTES ON THE INSTRUCTION MANUAL 7 4 3 ASSEMBLING THE EQUIPMENT 7 4 3 1 ASSEMBLING POSITIONING THE ENCLOSURE 7 4 3 2 HOW TO HANDLE THE PLANTAR TEST...

Страница 6: ...THE DATA OUTPUT TO MINIPRINTER 16 6 3 DATUM FORMAT 17 6 4 QUICK SETTING OF THE 37370 PLANTAR TEST 18 7 MEMORY MANAGEMENT 19 7 1 MEMORY RESET 20 7 1 1 MEMORY FULL MESSAGE 20 7 2 SETTING TIME DATE 21 8...

Страница 7: ...ETHOD PAPER 29 13 2 PAPERS MENTIONING UGO BASILE PLANTAR TEST 29 13 3 DURHAM HOLDERS FOR OROFACIAL STIMULATION 30 14 APPENDIX 1 FIGURE INDEX Figure 1 I R Source 2 Figure 2 Panel Mount Printer and USB...

Страница 8: ...h the Tail Flick method orofacial stimulation by optional holders see paragraph 5 5 Unique to the Plantar Test the animal is unrestrained and unhandled during experi ments which enables other more sub...

Страница 9: ...es over the sur face of the base platform see figure 1 At both sides of the handle 1 a START key is provided to help both left 2 and right 3 handed operators The cylinder contains a halogen bulb coupl...

Страница 10: ...20x20x14 h cm 2 3 Controller The controller front panel encompasses the COMMAND DISPLAY MODULE see para graph 2 3 1 and the slot for the memory key see paragraph 3 1 Data Portability The back panel s...

Страница 11: ...rre sponding key on the right of the display The right portion of the display window shows the abbreviation of the key function meaning OPR Operation FNC Function MEM Memory ESC Escape The display con...

Страница 12: ...port RS 232 C by the Delta 9 pin female connector COM 1 Communication is managed by the dedicated CUB Package Cat 52050 10 supplied as standard The CUB 52050 is a Windows based Data Acquisition Softw...

Страница 13: ...ional part suitable to print experimental data on 58mm wide paper see paragraph 12 3 Thermal MiniPrinter See paragraph 4 6 for connection to the Plantar Test via the 10 pin cable provided with the Min...

Страница 14: ...ng the Equipment Assemble the Plantar Test on a stable and flat bench or table surface Tighten the four supporting columns at the corners of the base platform until their upper right angle indentation...

Страница 15: ...during familiarization or test time A plantar surface if wet by urine will be definitely less sensi tive to the thermal stimulus because of the large specific heat of any watery solution see also par...

Страница 16: ...module the back panel embodies a connection module see figure be low Figure 4 Back Panel The connection module encompasses from left to right USB this USB type B connector for linking the Plantar Tes...

Страница 17: ...o ensure safe operation DO NOT attempt to open or perform any service work DO NOT connect up human subjects 4 10 Additional Safety Consideration a Place your Plantar Test on a flat surface b Pay atten...

Страница 18: ...The software version is necessary in case of communication with our laboratory for oper ational or servicing problems In case one of the captions MEMORY LOSS appears on the display please refer to pa...

Страница 19: ...be definitely less sensi tive to the thermal stimulus because of the large specific heat of any watery solution Be patient and dry the glass pane before the first test and at least between each test...

Страница 20: ...F3 NUL key After entering the last feature M or F the software goes automatically back to the main menu 5 6 1 Sequence Number The first additional information to be de fined is the sequence number Thi...

Страница 21: ...sed MALE FEMALE Select it by depressing the F1 UP or F2 DWN key and confirm it via the F4 OK key avoid the gender selection by depress ing the F3 5 6 4 Cut Off Time To get over a false start and to av...

Страница 22: ...ON window ap pears after some seconds select the de sired configuration by the F1 UP or F2 DWN key then confirm the selection by the F3 OK key Under the configuration name for example CONFIG01 CFG the...

Страница 23: ...C 6 1 How to set the Data output to PC The experimental data are saved in the instrument internal memory During the experi mental trial the data can also be routed to the PC for real time or remote co...

Страница 24: ...the F1 PRN is depressed the printer status changes from ENABLED to DISA BLED or from DISABLED to ENABLED 6 3 Datum Format When routed to the PC via the CUB or Win DAS Data Acquisition Software see th...

Страница 25: ...ion Manual extensively explains how to take advantage of all the features of fered by this microprocessor controlled unit The following table summarizes them how ever we recommend you to browse the co...

Страница 26: ...ical arrow keys F1 UP or F2 DWN to scan the data by momentarily depressing the key the displayed datum will increase or decrease of one meas urement Keeping these keys depressed longer will scan the d...

Страница 27: ...ppears also when the memory is fully loaded which means it has reached its maximum capabil ity of about 300 measures 7 1 1 Memory Full Message If the MEMORY FULL message appears during acquisition the...

Страница 28: ...e to obtain results as con sistent as possible In our case it is evident that 1 the positioning of the I R window in relation to the aimed stimulation area 2 the I R intensity are the parameters which...

Страница 29: ...on In fact the I R output of the Plantar Test may undergo to a certain reduction due to dust gathered on the optics blackening of the I R bulb accidental knocks ageing of components etc Moreover in ca...

Страница 30: ...hout I R Radiometer Calibrating the Plantar Test without the I R Radiometer means to recall the default cali bration value set at our factory during the testing phase From the Calibration Menu depress...

Страница 31: ...usted the dis play shows the caption MEMORY LOSS To replace batteries first of all switch off the unit and disconnect the mains cable N O T E when the caption MEMORY LOSS appears the internal memory i...

Страница 32: ...ph 2 1 To replace the bulb and in general to service the I R Source it is necessary to open the I R Emitter vessel use a 1 5 hex wrench to remove the 3M set screws located around the Vessel upper rim...

Страница 33: ...ng accidental knock may break the glass pane which is rather thin to minimize I R absorption losses Pay attention when handling the glass pane In case of breakage use heavy duty gloves to handle the g...

Страница 34: ...Commands via soft buttons Read out multifunction graphic display Print out Via optional thermal MiniPrinter or panel mount printer Starting via keys on the I R Vessel Additional key on the Controller...

Страница 35: ...age E WP 008 Mains Cable E FF 002 Set of 2 fuses 2A 6 3x32 52010 323 USB cable 12 1 Spare parts E HR 002 Spare I R Bulb Halogen Bellaphot Mod 64607 OSRAM 8V 50W E US 063 1 Battery Snaphat type M4T32 B...

Страница 36: ...acterization of the Foot Withdrawal Response to Noxious Radiant Heat in the Rat Pain 59 85 97 1994 13 2 Papers Mentioning Ugo Basile Plantar Test L Mannelli et alia Effects of the Neutrophil Elastase...

Страница 37: ...nstriction sciatic nerve pain hypersensitivity testing H N Kim et alia Electroacupuncture Inhibits Phosphorylation of Spinal Phosphatidylinositol 3 kinase Akt in a Carrageenan induced Inflammato ry Ra...

Страница 38: ...Page 31 37370 Instruction Manual Rev 0 _______________________________________________________________________ INSTRUCTION MANUAL October 2013 REVISION 0...

Страница 39: ......

Страница 40: ...ical problems which af fect temperature per se An objective way to quantify the intensity of the IR stimulus is to calibrate its power Power may be quickly and definitively be measured in mW per squar...

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Страница 44: ...anufactured in compliance with the following European Union Directives and relevant harmonized standards 2006 95 CE relating to electrical equipment designed for use within certain voltage limits 2004...

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