After each image adjustment:
a.
Touch
Apply
to accept the changes.
b.
(Optional)
Touch
Next
to display the analysis table. At the top of the table,
touch to change the table output by:
Output
Units
Description (Lane and Band Table)
Volume
Intensity
Sum of pixel intensities contained in an
identified band.
Weight
kDa
Molecular weight estimated for an unknown
sample based on the sample's relative
mobility in the gel versus the molecular
weight and relative mobility of known
standards.
Density
Intensity/Area Average intensity for all pixels contained in
an identified band (Volume divided by Area).
Local
Background
Corrected
Density
Intensity/Area Local Background Corrected Volume
divided by the Area.
Local
Background
Corrected
Volume
Intensity
Volume minus the local background
intensity (2-pixel area surrounding the
identified band region).
Migration
Distance
R
f
Distance migrated divided by the gel length.
c.
For TPN
‑
normalized data, the lane and band table has the following
additional outputs:
Output
Units
Description (Lane and Band Table)
Total Lane
Volume
Intensity
Sum of pixel intensities contained in an
identified lane.
Background
Volume (RB)
Intensity
Background determined using Rolling Ball
Background Correction.
Adjusted Total
Lane Volume
Intensity
Total Lane Volume minus background
volume.
Normalization
Factor
—
Normalization factors are proportional to the
total lane protein signal with a reference
lane nominally set to 1.000.
Area (band)
[1]
Pixels
Total number of pixels contained within an
identified band.
Normalized
Corrected (band)
Volume
[1]
Intensity
Adjusted Total (band) Volume divided by the
Normalization Factor of the lane.
[1]
This output is available in the report only.
Export and analyze images
Analyze images
iBright
™
CL750 Imaging System User Guide
25
