Use the Oligo calculator:
The oligonucleotide calculator determines the following data for a base
sequence that you enter:
•
Number of bases.
•
Percent GC content.
•
Molecular weight.
•
Absorptivity (extinction coefficient).
•
Conversion factor to be used in oligonucleotide measurements.
•
T
m
for oligos up to 20-mers, DNA, DNA hybrids and RNA hybrids.
1.
Highlight
Biological Tests
on the Main Menu page.
The Biological Tests sub-menu will open.
2.
Choose
Oligo Calculator
and press
Enter
.
64 Evolution 300 and Evolution 600 User Guide
Thermo Fisher Scientific