•
Enter one wavelength in Serial or Parallel mode. Multi wavelength
or Difference modes require two wavelengths. Highlight the
wavelength line and press
Enter
. Key in the required wavelength
and press
Enter
.
•
Enter the required Total Measurement Time in Serial,
Multi wavelength and Difference modes. In Parallel mode, the
Total Measurement Time is displayed but cannot be edited.
•
In Multi wavelength, Difference and Parallel modes, enter the cycle
time.
•
If a delay is required between the start of the run and the first
measurement, enter a delay time.
•
Choose whether to display the absolute values of the absorbance, or
the change in the absorbance relative to the initial measurement. If
Relative absorbance is chosen, an additional menu item appears to
set positive or negative slope.
•
Set up Graph High and Graph Low to accommodate the expected
absorbance range. The graph can be reconfigured post run, if
required.
•
Enter a factor and units, if required.
•
Smoothing may be specified at run time or applied post run.
•
Save the new method into the instrument Library or to disk. Press
the
Save Method
function key and complete the save dialog as
required.
52 Evolution 300 and Evolution 600 User Guide
Thermo Fisher Scientific
