•
Highlight
Standards
and press
Enter
to open the Standards Entry
page. Enter the concentration of each standard. Press the
Accept
function key when all standards have been entered.
•
There may be 1, 2 or 3 replicates per standard.
•
Normally, Measure Standards is set to Yes. When set to No,
absorbance values may be entered manually.
•
The minimum number of standards required will depend on the
curve fit.
Linear: 2
Linear through 0:
1
Quadratic: 3
Quadratic through 0:
2
Normally, a calibration contains more than the minimum number
of standards.
•
Timers and UV
calc
equations can be used, if required.
3.
Press the
Load Method
function key to load a previously saved
method from the instrument Library or from disk.
4.
Clear the beams or insert suitable blank(s) and close the lid of the
sample compartment.
5.
Press
Zero
to zero the instrument.
6.
Press the
Calibrate
function key.
Follow the on screen instructions, which will depend on whether a cell
programmer is in use and on the number of standards and replicates.
Thermo Fisher Scientific
Evolution 300 and Evolution 600 User Guide 49
