2.
Set up method parameters.
Parameter
Function
Measure Standards
Use Enter to toggle between Yes and No.
Yes - Enter the Ids of the standards and concentrations
into the Standards Table. After pressing the Calibrate
function key, the user will be prompted to measure
each standard.
No - Highlight the first standard ID in the Standard
Table and press Enter.
The Library page will open showing available .FXD
results files. Highlight the required Fixed
multiwavelength result file and press the Load File
function key. The method parameters will be set to
those used to measure the first standard and cannot be
changed.
All subsequent standards must have been measured
using exactly the same method. Each file must contain
data for a single sample. Use Clear Results between
Fixed measurements.
Standards
Transfers to the MCA standards page.
When Measure Standards = Yes, enter an ID and
concentration for each standard.
When Measure Standards = No, load Standards as
described above and enter a concentration for each
standard.
Wavelengths
When Measure Standards = Yes, enter the
wavelengths at which standards and samples will be
measured. Wavelengths may be entered from a scan.
When Measure Standards = No, the wavelengths used
are defined by the standard method
Note
At least one wavelength must be entered for each standard. Choose
wavelengths at which there are significant differences between standards.
3.
Clear the beams or insert suitable blank(s) and close the lid of the
sample compartment.
4.
Press
Zero
.
Thermo Fisher Scientific
Evolution 300 and Evolution 600 User Guide 55