70
intercalating dye such as SYBR
®
Green I is used, for confirmation that the correct product has
been amplified.
•
Click
Add Ramp
to open the ramp read parameters box:
•
Start Temperature:
Starting temperature of the ramp can be anywhere from 4°C.
•
End Temperature:
The temperature can be set to ramp to any temperature up to 98°C.
•
Temperature Increment:
The temperature can be increased in increments of between 0.1
and 5°C. The default is 0.5°C.
•
Hold Time:
The user can choose run the ramp with a set hold at each temperature; this can
be any time up to a maximum of 99:59:59 hrs:min:sec. The default is 10 seconds.
•
Max Heating Rate:
Allows the heating rate to be set between 0.1°C/sec and 2.2°C/sec.
•
Do Not Pause/Pause After Stage:
If set to pause, the run will pause after each stage; click
to change to Do Not Pause.
•
Number of Reads:
Displays the number of readings programmed for the ramp (maximum
of 4).
•
Number of Steps:
Displays the total number of steps for the ramp stage. This will depend
on the temperature range and increment.
•
Set the start temperature, the end temperature and the temperature increment between
reads. These can be changed either by using the sliding thermometer or by typing in the
respective box and clicking the enter key. The number of steps will be shown in the top-right
according to the settings chosen.
•
Set the hold time and choose whether to set the heating rate or to program in a pause.
•
Add a read to the ramp stage by clicking
Add Read
in the ramp read parameters box. The
same principles apply for setting up a read in a ramp stage as for a typical temperature step,
described in section 3.4.3.5.
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