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3.2.2
The Editors ................................................................................................................ 55
3.3
Using Quansoft ................................................................................................................... 57
3.3.1
Home page ................................................................................................................ 57
3.3.2
Main screen ............................................................................................................... 58
3.3.3
Navigation bar ............................................................................................................ 58
3.3.4
Title bar functions ...................................................................................................... 59
3.3.5
Menu bar functions .................................................................................................... 60
3.3.6
Accessing the editors ................................................................................................. 61
3.4
Setting up an experiment ................................................................................................... 62
3.4.1
An Overview .............................................................................................................. 62
3.4.2
Creating a new experiment ........................................................................................ 63
3.4.3
Setting up a program ................................................................................................. 64
3.4.4
Setting up a plate layout ............................................................................................ 74
3.4.5
Defining the analysis method .................................................................................... 83
3.4.6
Saving an experiment to the library ........................................................................... 87
3.5
Running an experiment ...................................................................................................... 89
3.5.1
Starting the run .......................................................................................................... 89
3.5.2
Monitoring the run ...................................................................................................... 92
3.5.3
Stopping or pausing a run.......................................................................................... 94
3.6
LED intensity settings ......................................................................................................... 95
3.7
Results Editor ..................................................................................................................... 96
3.7.1
Post-run analysis main screen .................................................................................. 96
3.7.2
Viewing the results of a run ....................................................................................... 97
3.7.3
Editing a graph ........................................................................................................... 99
3.7.4
Changing the analysis parameters .......................................................................... 100
3.7.5
Log/Audit trail ........................................................................................................... 102
3.7.6
Report ...................................................................................................................... 102
3.8
Exporting and printing results ........................................................................................... 104
3.8.1
Exporting .................................................................................................................. 104
3.8.2
Printing ..................................................................................................................... 105
4
Data analysis .......................................................................................................................... 107
4.1
Introduction ....................................................................................................................... 109
4.1.1
Amplification curve ................................................................................................... 109
4.1.2
Thresholds ............................................................................................................... 109
4.1.3
Fit points .................................................................................................................. 110
4.1.4
First derivative maximum ......................................................................................... 111
4.1.5
Standard curve ........................................................................................................ 111
4.1.6
Dissociation curve .................................................................................................... 111
4.2
Choosing an analysis method .......................................................................................... 113
4.3
Analysis method: None .................................................................................................... 115
Содержание PrimeQ
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