7. Quality Control
164
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
2016-04
Precision
Perform the following measurement to determine the precision/reproducibility:
Measurement Parameters:
Parameter
Setting
Reading Mode
Fluorescence Bottom
Ex Wavelength
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Em Wavelength
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
Number of flashes
25
Integration Time
40
Settle Time
0
Gain
Optimal
Plate Type
GRE96fb
Part of the Plate
A1
Kinetic
20 Cycles
Interval Time
Minimal
Plate Layout:
<>
1
2
3
4
5
6
7
8
9
10
11
12
A
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M
F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
B
C
D
E
F
G
H
Filling volume: 200 µl
Material/Reagents:
25 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate µClear, black with transparent bottom
200 µl P tips
Calculation of Precision:
