7. Quality Control
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
161
7.3.2
Fluorescence Bottom
For the Infinite 200 PRO with the option ‘Fluorescence Bottom’ the following tests
may be performed to prove the specifications:
•
Sensitivity (Standard)
•
Sensitivity (Enhanced)
•
Uniformity
•
Precision/Repeatability
These test instructions are valid for the Infinite 200 PRO:
•
Infinite F200 PRO
•
Infinite M200 PRO
•
Standard version
•
Spectrally enhanced version
Sensitivity (Standard)
Perform the following measurement to determine the detection limit for
Fluorescein:
Measurement Parameters:
Parameter
Setting
Reading Mode
Fluorescence Bottom
Ex Wavelength
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Em Wavelength
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
Number of flashes
25
Integration Time
40
Settle Time
0
Gain
Optimal
Plate Type
GRE96fb
Plate Layout
Pipette 200 µl of 25 nM fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
<>
1
2
3
4
5
6
7
8
9
10
11
12
A
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
25 n
M F
luor
es
c
ei
n
B
la
n
k
B
C
D
E
F
G
H
