7. Quality Control
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
159
Uniformity
Perform the following measurement to determine the Uniformity:
Measurement Parameters:
Parameter
Setting
Reading Mode
Fluorescence Top
Ex Wavelength
Infinite F200 PRO: 485 (20) nm
Infinite M200 PRO: 485 nm
Em Wavelength
Infinite F200 PRO: 535 (25) nm
Infinite M200 PRO: 535 nm
Number of flashes
25
Integration Time
40
Settle Time
0
Gain
Optimal
Plate Type
GRE96fb
Plate Layout:
Pipette 200 µl of 1 nM Fluorescein or the blank solution (0.01 M NaOH) into the
appropriate wells according to the plate layout:
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1
2
3
4
5
6
7
8
9
10
11
12
A
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
1 nM F
lu
or
es
c
ei
n
B
la
n
k
B
C
D
E
F
G
H
Material/Reagents:
1 nM Fluorescein (in 0.01 M NaOH) (Fluorescein sodium salt, Sigma F6377)
0.01 M NaOH (=Blank) (NaOH pellets, Merck article no. 6495 or Sigma S8045)
1 Greiner 96-well plate black
200 µl P tips
Calculation of Uniformity:
F
F
mean
100
*
stdev
(%)
Uniformity
=
mean
F
Average RFU value of wells filled with fluorophore
stdev
F
Standard deviation of RFU values of wells filled with fluorophore
The result of the formula determines the uniformity in % CV.
