5. Operating the Instrument
2016-04
IFU for Infinite 200 PRO No. 30052730 Rev. No. 1.6
105
In addition, the plate type (e.g. material of bottom of the microplate) also
influences the characteristics of the light beam. Therefore the theoretical border
displayed in the software might not correspond to the actual border when
measuring a real sample. It is therefore strongly recommended to optimize the
Multiple Reads per Well parameters for every new application. Make sure that the
selected border ensures sufficient distance between light beam and wall of the
microplate well.
CAUTION
ALL ABSORBANCE AND FLUORESCENCE INTENSITY
SPECIFICATIONS GIVEN IN THIS DOCUMENT ARE ONLY VALID FOR
SINGLE POINT MEASUREMENTS (ONE MEASUREMENT POINT PER
WELL). WHEN USING THE MULTIPLE READS PER WELL OPTION THE
SPECIFICATIONS ARE NOT VALID.
CAUTION
THE SOFTWARE DISPLAYS ONLY A SCHEMATIC VIEW OF THE
MEASUREMENT PATTERN. THEREFORE OPTIMIZE THE MULTIPLE
READS PER WELL PARAMETERS FOR EVERY NEW APPLICATION.
MAKE SURE THAT THE SELECTED BORDER
IS SUFFICIENT TO AVOID AN OVERLAP BETWEEN THE LIGHT BEAM
AND THE WELL WALL OF THE MICROPLATE.
CAUTION
A ‘BORDER’ VALUE THAT IS TOO SMALL MAY CAUSE
WRONG MEASUREMENT RESULTS DUE TO OVERLAP BETWEEN THE
LIGHT BEAM
AND THE WELL WALL OF THE MICROPLATE.
STOP
STOP
STOP
