SynOptics SYNBIOSIS aCOLyte3, Руководство пользователя

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Using Measurement Mode to count
colonies
Once you have created and accepted a new batch (see Creating a new batch , page 17, and
Accepting the batch design , page 56), the general procedure for counting colonies is:
1. Open or select the batch – see the next section, Opening and selecting batches .
2. Load the first plate into aCOLyte3 – see Loading plates into the instrument , page 8.
3. Capture an image – see
Capturing an image
, page 9.
4. Adjust the measurement frame(s) if required – see Adjusting frame settings in
Measurement mode , page 63.
5. Enter a plate identifier if it has not been entered automatically, or change an
automatically entered identifier and dilution if required – see
Entering plate identifiers
and dilutions , page 63.
6. Carry out the measurement – see Counting colonies , page 68.
7. Repeat Steps 2–6 for all the other plates in the batch.
8. Check the results and edit them if necessary, then create a report for the batch – see the
following chapter, Working with results , page 77.
9. Close the batch – see Closing batches , page 75.
Steps 2 and 3 are also required when creating and setting up the batch – the cross references
take you back to earlier sections in the Manual. Step 8 is described in the next chapter,
Working with results
, page 77.
Instructions for the remaining steps are given in this chapter (though much of Step 4 is
common to creating and setting up the batch and is covered by references to the
Creating a
new batch , chapter).
Opening and selecting batches
If you have just created and accepted a new batch (see Creating a new batch , page 17, and
Accepting the batch design , page 56), it will be automatically open and selected in
Measurement mode. However, you can also open a previously created batch if there is no
batch open currently, or if you want to open another batch in addition to any currently open
batches (you can have several batches open at the same time – see later in this section for
how to select which open batch to work with).
To open a batch in Measurement mode:
1. Press:
aCOLyte3 User Manual 61