QIAsymphony SP/AS Consolidated Operating Guide 12/2017
132
12.4.2
Defining/checking sample rack(s)
Once rack file(s) and rack type(s) have been assigned to “Sample” slot(s), the positions of
samples and controls and the associated volumes must be defined.
1.
Press
Next
in the
Sample Rack(s)
screen.
2.
The
Sample Rack Layout
screen appears.
This screen displays a schematic of the sample rack in the selected “Sample” slot. If two “Sample”
slots were defined, the
Slot 1
and
Slot 2
buttons can be used to switch between views of the two
“Sample” slots.
If rack file(s) were assigned, sample positions, extraction controls, and volumes are already
defined and are displayed in the sample rack layout. It is only possible to modify the sample
volumes. This may be necessary if some eluate was manually removed from the rack before being
placed on the QIAsymphony AS. It is not possible to define additional sample positions.
If rack file(s) were not assigned, sample positions, control positions, and volumes must be
manually defined. When a rack file has not been assigned it is also possible to edit the sample
IDs.
Important
Samples and/or extraction controls that have been processed on the QIAsymphony
SP and marked as “invalid” are marked in red. These “invalid” sample and
extraction controls cannot be processed by the QIAsymphony AS and cannot be
selected by the user in the
Assay Assignment
screen. In the
Assay Assignment
screen,
any “invalid” samples appear as an empty well.
Important
If an assay rack file is being used as a sample rack file the abbreviations used
for assay standards (Std), no template controls (NTC, NTC+IC, NTC–IC; where
IC is internal control), and assay controls (AC) are not displayed — only the
volume is visible. Press the position (pale yellow) to select it, and then select
Sample
,
EC+
or
EC–
to define the sample type (where EC is extraction control).
The
Next
button becomes active after sample positions and volumes have been assigned to the
sample rack.
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