ProteOn XPR36, Руководство пользователя

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ProteOn XPR36 | Experiments
68
Preparing the Samples
The following sample preparations are general workflow instructions. This
procedure assumes that the ProteOn XPR36 instrument is configured with the
ProteOn sample rack. Adapt these instructions for use with a ProteOn
microplate if the instrument is configured with microplates. Samples should
be particulate free and degassed.
To prepare the samples:
1. Dissolve and aliquot the ProteOn EDAC and sulfo-NHS. See To set up a
sample holder: on page 69.
2. Remove the required quantities of EDAC and sulfo-NHS from the
freezer and keep them on ice.
3. Remove the ligand and analyte samples from the freezer or refrigerator.
If the solutions are frozen, allow them to thaw.
4. Centrifuge all sample vials for about 15 sec to ensure that all of the
solution is at the bottom of the tubes. Place the vials on ice, if required.
5. Print out a sample layout report for the experiment. See To generate a
protocol or sample report: on page 62.
6. Label the appropriate number of ProteOn sample vials as described in
the sample layout report.
7. Dilute the analyte and ligand as required for the experiment, and place
the samples at the location specified in the sample layout report.
8. Prepare all other reagents required for the experiment. Place the
reagents in the sample rack location specified in the sample layout
report.
9. Mix the thawed EDAC and sulfo-NHS solutions immediately before use.
These reagents react rapidly with each other, resulting in an activity
decrease over time.
10. Place the solutions inside vials in the appropriate rack positions.
Caution: Do not mix the EDAC and sulfo-NHS
solutions until the experiment is ready to start.