ProteOn XPR36, Руководство пользователя

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ProteOn XPR36 System | Protocol Design
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See also Experiments with Highly Refractive Cosolvents (DMSO) on page 84.
You can also use this option to determine the effects of buffer on an
interaction. Once the buffer valve is switched, the protocol must pause and
wait for the selected buffer to be flushed through the fluidics system. Each
time the buffer valve is changed, the SPR reference curve is automatically
recalculated to obtain optimal results.
To set the buffer step:
• In the Protocol Editor panel, select the Set Buffer step in the Step
list. Existing or copied protocols maintain the buffer valve position
specified in the original protocol.
The details appear in the Step Details panel.
When the Set Buffer step executes, the buffer valve position changes to
the buffer position you specified and the instrument goes through the
flush operation. When the instrument completes the flush operation, a
15 sec delay takes place, and the new buffer flows through the flow cell.
At the end of the delay, the SPR curve is recalculated.
Setting the Temperature Step
Use the Set Temperature step to change the chip/flow cell temperature in the
ProteOn™ XPR36 system during an experiment when the instrument is
unattended. When the temperature change request is sent, the protocol
pauses until the chip reaches the new temperature.
Because SPR is more sensitive to temperature change than is the
thermocouple used to measure the temperature, the Set Temperature time will
be reached before a stable SPR baseline is obtained. Therefore, it is important
to wait for an additional period of time after the set temperature has been
reached. We recommend that you wait 45–60 min for a 5°C temperature
change.
Note: The Set Temperature step does not change the
autosampler sample temperature.