ProteOn XPR36, Руководство пользователя

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Langmuir Model 1:1 Acquired SPR sensorgram data are to be analyzed using a
mathematical equation or model for the interaction. For a Langmuir
model, the reaction between immobilized ligands (L) and analytes (A)
is assumed to follow a pseudo first-order kinetics. The Langmuir
model also assumes that the binding is equivalent and independent
for all binding sites (1:1) and that the flow in the cell is sufficiently
high so that there is no depletion of or accumulation of analyte in
solution, and so that the analyte concentration remains constant
Langmuir with Drift Langmuir model with an extra parameter for fitting data with linear
baseline drift
Ligand In the ProteOn XPR36 system, the biomolecule immobilized on the
sensor chip surface. In general, ligand can refer to any molecule that
covalently binds to the chip surface (for example, EDAC, sulfo-NHS,
ethanolamine, protein, DNA, etc.)
Local Scope assigned to a parameter, meaning that a value for each
sensorgram will be fitted
Mass Transport Process of an analyte diffusing from the bulk solution to the
biosensor chip surface. An interaction is said to be "mass transport
limited" if the analyte binds to the ligand on the chip surface faster
than it can diffuse from the bulk solution to the chip surface
Matrix Effect Change in the molecular layers on the sensor chip surface that
produces an SPR signal change or response
Microfluidics
System
Component of the fluidics system that directs and regulates the flow
of small amounts of buffer and samples through the flow cell. It is
composed of the autosampler, syringe pumps, degasser,
multichannel module, valves, and associated tubing
Microplate Sample container with 96 wells that are formatted in a 12 x 8 array
suitable for parallel sample processing
Multichannel
Module (MCM) Component of the microfluidics system that, when pressed on the
sensor chip surface, creates six parallel channels through which
ligand- or analyte-containing solutions can flow
Needle Holder Component of the autosampler that holds the six needles in the
proper configuration for delivering samples from the sample holder
into the microfluidics system
NHS N-hydroxysuccinimide is used for activation of the sensor chip
surface (see
Activation )
Nonspecific Binding Binding (by an undesired mechanism) of analyte, ligand, or any
contaminants found in any fluid flowing over the sensor chip surface