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Index

165

Index

2

21 CFR Part 11, 12

A

Account lockout, 157
Active protocols, 24
Administrator, 153
Advanced level, 68, 121
Aperture slide, 73, 77, 80, 83, 87
Archive, 160

Archive old results, 161

Archive old results, 161
Audit Trail, 158

Archive button, 159
Export button, 159
Protocol button, 159

Auditor, 153
Automatic logout, 129

B

Barcode, 22, 27, 28, 29, 31, 33, 53, 122
Barcode options, 22, 122
Barcode reader option, 22
Beam size (LANCE), 88
Beam size (Time-resolved fluorometry), 74
Button light, 33
Button on Wallac 1420, 33
Buttons (Measurement tab), 45
Buttons in the filters window, 90
Buttons in the Labels window, 69
Buttons in the Misc. settings window, 118

C

Change password, 18
Change the CW-Lamp, 97
Changing the CW-Lamp filter wheel, 97
Changing the emission filter slide, 95

Column width changing, 59
Columns of wells, 120
Communication Settings, 125
Constant voltage mode, 76
Control modes

Constant voltage mode, 76
For fast kinetics, 76
For fluorescence polarization, 76
Stabilized energy mode, 76

Counter position (Fluorometry), 77
Counting parameters (LANCE), 88
Counting parameters (Time-resolved

fluorometry), 74

CW_Lamp control mode, 76
CW-Lamp changing, 97
CW-Lamp energy (Fluorescence

Polarization), 83

CW-Lamp energy (Fluorometry), 76
CW-Lamp filter wheel, 96
CW-Lamp filters, 76, 78, 83, 92

D

Define labels, 69
Delay button (Measurement tab), 46
Delete button (Measurement tab), 45
Demo, 19
Demo mode, 121, 122
Dimensions of a plate, 120
Dispense button (Measurement tab), 45
Dispenser information, 143
Dispenser maintenance, 67, 147, 151

Needles, 151

Dispenser unit, 11
Display problems, 134
Down button (Measurement tab), 45
Dual label, 39
Dual label normalization, 100
Dual label normalization wizard, 100

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Содержание Wallac 1420

Страница 1: ...March 2003 User manual 1420 Software version 3 00 PerkinElmer Life and Analytical Sciences Wallac Oy P O Box 10 FIN 20101 Turku Finland Tel 358 2 2678111 Fax 358 2 2678357 Website www perkinelmer com...

Страница 2: ......

Страница 3: ...eration with the Start button 23 Starting operation with the Start Wizard 26 Starting operation and using a protocol number barcode 28 Stacker operation 29 Starting operation no protocol number barcod...

Страница 4: ...urement 47 Plate 48 Plate repeat parameters 48 Measurement height 48 Plate type 49 Temperature level checking 49 Outputs 50 Events 52 Keywords 53 General 54 File menu 55 Save 55 Print 55 Print preview...

Страница 5: ...s for the main counting window 74 Beam size 74 Second measurement parameters 74 Fluorometry 75 Protocol name 75 CW Lamp energy 76 CW Lamp Control Mode 76 CW Lamp filters 76 Excitation aperture Wallac...

Страница 6: ...mission aperture 83 Counting time 83 G factor 84 LANCE 85 Protocol name 85 Flash Energy Area 86 Flash Energy Level 86 Excitation filter 86 Light integration capacitors 86 Note 87 Light Integration Ref...

Страница 7: ...warning page 102 Normalization confirmation page 103 LANCE normalization 104 Using the LANCE normalization wizard 104 Protocol selection 105 Preparing the plate 106 Measurement status page 107 Measure...

Страница 8: ...t met 135 Appendices 137 Appendix 1 Connecting MultiCalc to Wallac 1420 139 Introduction 139 Wallac 1420 MultiCalc assay protocols 139 Installation of Wallac 1420 output option in MultiCalc 140 MultiC...

Страница 9: ...s 153 User Management 154 Password Control 156 Audit Trail 158 Archive 160 Security Settings 161 Index 165 Trademarks DELFIA is a registered trademark and LANCE is a trademark of PerkinElmer Inc Windo...

Страница 10: ......

Страница 11: ...9 Introduction...

Страница 12: ...10...

Страница 13: ...ensitivity time resolved fluorometry homogeneous time resolved fluorometry LANCE option and photometry It is a compact bench top unit with features such as dispensers shaking temperature control top o...

Страница 14: ...requirements set forth in agency regulations i e GMP GLP GCP It also applies to electronic records submitted to FDA under requirements of the Federal Food Drug and Cosmetic Act The Enhanced Security...

Страница 15: ...tem and Windows administrator rights See Appendix 4 for more details 1 Load the software CD Load CD 2 The setup loader will start automatically and give you a choice of either selecting the Installati...

Страница 16: ...set using the protocol editor This is activated by selecting a protocol in the Explorer A sample plate can then be loaded and measured using the protocol set for it Other parameters affecting the whol...

Страница 17: ...15 Operating Wallac 1420...

Страница 18: ...16...

Страница 19: ...up Wallac 1420 Without enhanced security mode When you boot the system the following window appears With enhanced security mode When the Enhanced Security mode is installed the login dialogue appears...

Страница 20: ...s three tabs Instrument Control Live Display and Temperature The instrument control tab enables you to control the instrument by starting stopping or ending counting It shows you the protocol being us...

Страница 21: ...shows you the live results in the form of a plate map with colour coding to indicate the size of the results If you move the cursor on to a well that has been measured the numerical value will appear...

Страница 22: ...g beside the plate map Choose the range that is appropriate for the samples you are measuring so that you get the greatest variation in sample colours You can choose whether you want the scale to be l...

Страница 23: ...ew value is only effective when the Apply button has been clicked The figure shows the temperature rising rapidly to its new level and then stabilizing there If the plate heating option is not install...

Страница 24: ...sing stackers In this case the first plate to be loaded from the stacker must have a barcode Measurement can be started simply by briefly pressing the button on the counter See page 118 for more detai...

Страница 25: ...ng Wallac 1420 23 Starting operation with the Start button 1 Open the loading position lid 2 Load your first plate and close the lid See the picture on page 20 for correct plate orientation Load first...

Страница 26: ...4 3 Select the protocol you want to use by scrolling through the list of active protocols 4 Use the mouse to click the Start button on the user interface You can follow the measurement process by sele...

Страница 27: ...measured plate 2 Load the next plate 3 Press the button briefly 6 Briefly press the button on the counter this will be blinking if there are more plates to be loaded for this assay If it is not blink...

Страница 28: ...e Operator user group An alternative way to start counting is to use the Start Wizard This allows you to define how many plates you are going to use and to give the positions of samples Proceed as fol...

Страница 29: ...t definition will lead to incorrect results Comments that you want added to the results just type in what you want Load the first plate the protocol name and comments appear in order to confirm that i...

Страница 30: ...ified by the barcode will be selected and used for measuring the plate When the measurement has finished and the light starts blinking again remove the plate and load the next one and briefly click th...

Страница 31: ...y output stacker the one with the tabs for the quick release mechanism should be fitted first into the multilabel counter it occupies the left hand position 3 Fit the input stacker with plates in the...

Страница 32: ...Start as described above 6 When all plates have been moved to the output stacker it can be removed To do this press the release tab at the bottom of the stacker then lift it up After you have removed...

Страница 33: ...e If it has no barcode it will be measured using the protocol specified by the previously read barcode 3 Only when the input stacker is empty will operation stop The button light will be blinking Remo...

Страница 34: ...asured is brought out to the loading position You cannot resume measurement after clicking Stop but must begin the assay from the beginning if required End button Clicking the End button in the workst...

Страница 35: ...no assay is running and you press the button for more than 2 s then a new assay will be started with the current protocol This will not work if the protocol barcode option is selected or the enhanced...

Страница 36: ...Operating Wallac 1420 34...

Страница 37: ...35 Protocol editing...

Страница 38: ...36...

Страница 39: ...hanced security mode Explorer is enabled only for users who belong to the Editor user group Through the Explorer you can access the Protocol Editor and the Result Viewer Click the Explorer icon to ope...

Страница 40: ...the mark next to the folder to see the protocols contained in it To close a folder either double click on it or click the sign next to it If you are working on the Advanced or Service level you can c...

Страница 41: ...ave it e g as a user protocol in the Users folder or you can save it according to the technology The choice is yours but the default structure gives you a starting point and helps you to find the pre...

Страница 42: ...o be followed after an assay has been run with this protocol General information about the protocol You can set parameters for up to 40 plates in one protocol Protocol entries are checked for validity...

Страница 43: ...ed as Measured and will become the last plate in the sequence When you delete a plate it is the one currently visible that is deleted A duplicated plate is a copy of the current plate and will become...

Страница 44: ...be selected by clicking the mouse and dragging in the same way as when selecting free well areas The whole plate can be selected by clicking on the upper left corner of the plate frame To select a re...

Страница 45: ...umber can be selected from a drop down list of available numbers The protocol name has to be typed in In the case of a factory pre set protocol you cannot change the number Notes or additional comment...

Страница 46: ...ou to specify on what unit the complete measurement sequence should be performed before moving on to the next similar unit The possible unit is a single well a strip or a plate Plate and strips modes...

Страница 47: ...oves the selected operation in the operation list one step closer to the beginning This button is enabled when the operation currently selected in the measurement operations list is not the first oper...

Страница 48: ...he first of the plate repeats check this box If you want delay for every repeat then leave it unchecked Shake button Clicking this causes a dialogue to appear that lets you specify the parameters for...

Страница 49: ...ber of horizontal steps the number of vertical steps and the point displacement i e the distance between each point on the well where the measurement is made The total number of points can be a maximu...

Страница 50: ...give the time between the end of one measurement and the beginning of the next 0 3600 secs This allows you to do slow kinetic measurements Measurement height Standard This selection is only enabled wh...

Страница 51: ...ke sure you select the plate type before defining the plate map to avoid losing your settings Plate types are read from the Wallac 1420 database when the Protocol Editor is started so it will not reco...

Страница 52: ...t you must select the format either plate format results are in a table format with rows and columns in the order shown in the plate map and or list format results are in a single column starting with...

Страница 53: ...are the same as for printer output List Plate Protocol description Error description Notes You must also type in the file name This can include keywords connected with the file as described in Events...

Страница 54: ...n while the second box specifies the command line arguments to use The button to the right of the first box lets you browse to find the executable file you want to use The button to the right of the s...

Страница 55: ...e on a plate you should define an explicit file name for the plate Output file name the name of the output file given in the Outputs tab In some cases the numeric value of the AssayID or the RunID may...

Страница 56: ...t was created and edited and when an assay was last run with it The user names used come from the identifier given when Windows was booted Pre set protocols are created in the factory In the enhanced...

Страница 57: ...ly you can zoom out reduce the magnification if the page is already magnified You can step to the next page or back to the previous one When you have finished viewing the pages that are going to be pr...

Страница 58: ...k it then whenever you click the Start icon measurement of the selected protocol will begin directly If you do check it then whenever you click the Start icon the Start Wizard will be used to start th...

Страница 59: ...57 Result viewing...

Страница 60: ...58...

Страница 61: ...ent assay running the latest assay run is displayed on the Viewer Results in the Explorer Alternatively once results have been received and stored in the computer you can view them by means of the Wal...

Страница 62: ...these described below The Result Viewer also provides you the following functions by means of command line menus or in most cases buttons File Export Button Page setup Print Button and Exit View List...

Страница 63: ...nt time and the other the result value You can change column widths to get more information In the case of high density plates results can be split over several tabs View all result data This item in...

Страница 64: ...n about the currently displayed plate The information included is Plate The number of the physical plate currently being displayed Repeat The number of the repeat of the physical plate currently being...

Страница 65: ...e plate grid below it The information included is Name of the label or result type displayed in the result plate Unit for the results from the result plate Instrument Background value measured for the...

Страница 66: ...rows of one plate will be printed on one page If you press OK in the print dialogue the selected parts of the run information will be printed on the printer that has been selected in the print dialogu...

Страница 67: ...65 Tools menu...

Страница 68: ...66...

Страница 69: ...fining filter types EuSm dual label normalization wizard requires TR FIA to be installed LANCE normalization wizard requires LANCE to be installed Plate dimension wizard for fine tuning the plate posi...

Страница 70: ...allows you to select the user level Routine Advanced or Service If the User Level is selected to be Routine this will automatically disable any system operations To enable these operations you must s...

Страница 71: ...be freely defined by the user but names of factory pre set labels cannot be changed See the sections below for details There are four buttons at the bottom of the labels window Add add a new item to...

Страница 72: ...ems and labels used in protocols cannot be removed This command requires confirmation because you cannot undo Remove Properties select an item to activate this View the properties of the selected item...

Страница 73: ...lved fluorometry a flash lamp is used to illuminate the sample different lanthanides are used as labels The picture shows the parameters that can be set Protocol name This is the name by which the pro...

Страница 74: ...igh values e g 255 along with 384 well or higher plates The reason is that this high power continuous usage combination may damage the older type of flash lamp The cooled flash excitation system avail...

Страница 75: ...lt viewer by selecting View all result data Emission filters You can select the emission filter from the drop down list of available filters See the chapter on Filters for more information about how t...

Страница 76: ...cle Eu 400 400 1000 Sm 50 100 1000 Tb 500 1400 2000 Dy 30 30 1000 Factory set values for the second counting window are always 0 0 The counts in this window are displayed only if the values deviate fr...

Страница 77: ...rum of a continuous wave lamp and used to excite the fluorochrome in the sample The parameters that can be set for fluorometry are the following Protocol name This is the name by which the protocol is...

Страница 78: ...lized energy mode This enables optimum performance in cases where the light intensity is critical as in fluorescence polarization or where speed is critical as in fast kinetics CW Lamp filters You can...

Страница 79: ...shutter is used at all times when no measurement is occurring to prevent stray light getting to the photomultiplier tube Counter position The counter position can be selected to be Top or Bottom Count...

Страница 80: ...sociated with it to prevent parameters being changed by an unauthorized person Absorbance mode Select whether you want visible light or ultraviolet absorbance Flash Lamp Filter These option buttons ar...

Страница 81: ...izes of excitation aperture controlling the size of the incident light beam You can select with the option buttons Small Normal or Large Normal is the default Reading time This parameter determines ho...

Страница 82: ...can be associated with it to prevent parameters being changed by an unauthorized person Emission filters You can select the emission filter from the drop down list of available filters See the chapter...

Страница 83: ...his aperture is used for the top fluorometry factory set labels There is also a large circular aperture 5 mm in diameter The shutter is used at all times when no measurement is occurring to prevent st...

Страница 84: ...e incident polarization S plane and one perpendicular to it P plane These are each in turn moved automatically into the emission light path The ratio 1000 Parallel G Perpendicular Parallel G Perpendic...

Страница 85: ...larizer in the excitation light path You can select by option buttons whether this is Small for high density plates or Normal 96 or 384 well plates Emission filter You can select the emission filter f...

Страница 86: ...lar S and P counts are background subtracted fluorescence count rates obtained from a pure fluorophore solution e g CorrectedS averageS 1nM averageS buffer and CorrectedP averageP 1nM averageP buffer...

Страница 87: ...donor molecule which after a delay transfers the energy to the accepter molecule which then emits light Two labels must be defined one for the donor and the other for the accepter The picture shows t...

Страница 88: ...igh values e g 255 along with 384 well or higher plates The reason is that this high power continuous usage combination may damage the older type of flash lamp The cooled flash excitation system avail...

Страница 89: ...lt viewer by selecting View all result data Emission filter You can select the emission filter from the drop down list of available filters See the chapter on Filters for more information about how to...

Страница 90: ...0 100 0 1000 High count 665 50 0 100 0 1000 Long decay 615 50 0 100 0 3000 Long decay 665 50 0 100 0 3000 545 50 0 100 0 3000 615 50 0 100 0 1000 665 50 0 100 0 1000 572 50 0 100 0 3000 TruPoint 615 5...

Страница 91: ...rate LANCE Long Decay 615 same as LANCE 615 but 3000 cycle time This label is for long decay Eu chelates for example Eu W8044 in a TR FRET assays LANCE Long Decay 665 same as LANCE 665 but 3000 cycle...

Страница 92: ...e name and edit the properties It will appear with a new icon showing it is different from a default item Copy select an item to activate this not available for slides Make a copy of an item It will a...

Страница 93: ...for its uses can be defined There is also a section where you can add additional information that you can connect with the filter properties Factory pre set emission filter names begin with a letter...

Страница 94: ...me of the filter and the technology for its use can be defined There is also a section where you can add additional information that you can connect with the filter properties Factory pre set CW lamp...

Страница 95: ...two types of slide Emission filter and CW lamp excitation filter The former is a strip and the latter a wheel The instrument is loaded with slide A in each case but an empty slide B is also available...

Страница 96: ...ved fluorescent chelates if this option is included position 5 and 6 for emission filters for fluorometry labels umbelliferone and fluorescein respectively Position 7 is kept open for luminometry meas...

Страница 97: ...ar telling you to unscrew and remove the plate on the side of the counter When you have removed the plate click OK to cause the slide to be ejected Pull the slide out carefully Avoid touching the filt...

Страница 98: ...efault for fluorometry and three for photometry The remaining positions can have filters added by the user Note default filters can be removed and replaced by user filters There is also a second filte...

Страница 99: ...eel and make sure you close the cover before starting a measurement Changing the CW Lamp If the CW Lamp needs replacing lift the cover to get access to the lamp Make sure the lamp is switched off and...

Страница 100: ...e emission filter slide is a strip To move a filter from the list to a place on the slide move the cursor to the filter name on the list drag it keeping the mouse button pressed down until it is over...

Страница 101: ...led D615 615 nm ca 8 5 nm ca 80 Europium chelate emission LANCE option installed D665 665 nm ca 7 5 nm ca 80 Samarium chelate emission D642 642 nm ca 6 5 nm ca 80 Terbium chelate emission D545 545 nm...

Страница 102: ...e a wizard that will guide you through the normalization procedure First comes a welcome to the wizard page You can select that this does not appear in the future After this the main steps in the norm...

Страница 103: ...g the plate The wizard explains what samples you should put in the plate and where you should put them Plates must have 3 or more strips maximum of 8 with 1 12 wells per strip The strips must be horiz...

Страница 104: ...n completed When the measurement is completed click Next to move the wizard directly to the Normalization confirmation page otherwise the wizard moves to the Measurement warning page Measurement warni...

Страница 105: ...e normalization ratio the actual samarium counts in the samarium window can be calculated by subtracting the europium contribution to the measured counts Additional information about the reliability o...

Страница 106: ...orrects for the following effects in LANCE measurements background from the plate crosstalk of the donor signal into the acceptor window and quenching The final result is normalized to the maximum don...

Страница 107: ...Tools menu 105 Protocol selection You must select from the list of LANCE protocols the one you want to do the normalization for Highlight the one you want and then click the Next button...

Страница 108: ...sstalk samples i e donor only samples in A3 and A4 these are used to calculate the amount of the blank corrected donor signal in the acceptor window as a proportion of the donor signal Only donor and...

Страница 109: ...This is shown during the actual normalization measurement and it gives you feedback about the measurement The measurement status pane shows the current status of the measurement The progress of the me...

Страница 110: ...nt warning page Measurement warning page This is used to inform you that something out of the ordinary has happened during the measurement of the normalization assay This page gives you enough informa...

Страница 111: ...r signal in the acceptor window and the maximum donor signal in the donor window These values are then used in a measuring sample to obtain the actual acceptor value i e the blank and crosstalk correc...

Страница 112: ...ine the protocol make sure that both LANCE labels are defined in the Measurement tab as shown below If only one label is selected then the measurement will be treated as normal time resolved fluoromet...

Страница 113: ...sed to make the necessary corrections for this particular LANCE measurement They will overrule any values that may have be stored in the instrument memory from a normalization with the LANCE wizard Ho...

Страница 114: ...ensity plates 384 or higher to ensure exact positioning of the plate The factory default plates cannot be changed If the same plate type is used for more than one technology it is recommended that you...

Страница 115: ...ick in the Plate name field to select the plate When you have done this Next will be enabled Click it to go to the next dialogue Technology selection You must select the technology to be used to count...

Страница 116: ...re the plate by dispensing appropriately labelled sample to each of the four corner wells of the plate All the other wells should be empty Specify the area of the well to be scanned The example shows...

Страница 117: ...Tools menu 115 When the plate has been prepared load it and click Next After scanning is completed the Next button will be activated Click it to continue Define the centre point of the corner wells...

Страница 118: ...s move the mouse cursor and click the left button again Repeat this for each of the four wells When the centre of all four wells has been marked the Next button will be enabled Click this to continue...

Страница 119: ...a number of default types Well names the available names are listed There are up to five default types two Empty and to be Measured that are generally valid and three LanceBlank LanceCrosstalk and La...

Страница 120: ...eceded by the words Copy of You can give it a different name and edit other parameters by selecting Properties Remove select a user created item to activate this Remove the selected item from the list...

Страница 121: ...s to see the plate properties In the case of Properties the dialogue that appears gives information about the plate Each of the parameters in this dialogue must be defined when you add a plate The sof...

Страница 122: ...t dimensions are needed to define a plate The units are millimetres and you can specify the dimension to the nearest hundredth of a millimetre Note the distances should be measured from the outermost...

Страница 123: ...ecurity mode this can be accessed if you belong to the Service user group All the four tabs plus the Automatic logout tab are shown General You can define the number of results to be held in the datab...

Страница 124: ...have a barcode then the protocol used to measure the previous plate will be used See the Plate orientation picture on page 20 for where to fix the barcode You can specify what happens when the end of...

Страница 125: ...es as from the same assay because each will have been produced by the same protocol If you are not using stackers the instrument waits until the button on the instrument is pressed and then begins mea...

Страница 126: ...selected in User Level In the enhanced security mode this tab can always be accessed It shows which options are installed These include Stacker option Barcode reader options Temperature control Adjust...

Страница 127: ...ction of this tab tells what measurement technologies are installed and available Fluorometry FP Fluorescence polarization Luminometry Photometry TR Fluorometry dissociation enhanced time resolved flu...

Страница 128: ......

Страница 129: ...127 Exiting...

Страница 130: ...128...

Страница 131: ...not possible In the enhanced security mode the File menu also contains a Logout menu item This allows you to log out from the workstation software You need to confirm the operation After the logout th...

Страница 132: ...Exiting 130...

Страница 133: ...131 Troubleshooting...

Страница 134: ...132...

Страница 135: ...the run Please try one or more of the following to correct the situation Check that Windows is set to handle your virtual memory settings Do the following to check this Open the Control Panel in Setti...

Страница 136: ...upgrade Performance degradation If you find your Wallac 1420 software is not running as fast as it used to please run the Wallac 1420 Database Maintenance program You should anyway do this on a regul...

Страница 137: ...communicate with the Instrument server if that is the case When you click the OK button the reboot sequence is activated and the PC will be restarted System requirements not met This dialogue will be...

Страница 138: ...Troubleshooting 136...

Страница 139: ...137 Appendices...

Страница 140: ...138...

Страница 141: ...he dialogue that appears select the Misc tab and then in the Background field make sure that Always suspend is not checked See also Installation in the Instrument Manual for how to install this option...

Страница 142: ...for Automatic evaluation whenever an assay file is available Property O is for the connection type Offline MultiCalc assay protocols All assays with Wallac 1420 have to be of the FLUORO type for all m...

Страница 143: ...ates C122 105 Streptavidin coated plates 1450 510 Rigid sample plate 1450 410 Rigid sample plate 1450 514 515 Isoplate Nunc 475515 FluoroNunc Black strip plate 437591 FluoroNunc White strip plate 4516...

Страница 144: ...550 Combiplate white 9502817 Cliniplate black Acceptable range of plate dimensions for plates in a 1420 216 Stacker Length 127 2 128 2 mm Width 84 5 86 0 mm Height 14 0 25 0 mm Ledge height 1 5 6 5 mm...

Страница 145: ...rposes All the needles are directed to the same well allowing dispensing of more than one reagent to the same well The number of pumps fitted and the total volume of each pump are set during installat...

Страница 146: ...te vial Note the waste vial must be empty when operation starts To check that the waste vial is empty and if not to empty it proceed as follows Open the top and front cover of the dispenser and then l...

Страница 147: ...y out any liquid following the disposal procedure recommended by the manufacturer of the reagent you have been using Then replace the waste vial on its holder and slide it back into place Replace the...

Страница 148: ...Appendix 3 Dispenser operation 146 Preparing the tubing Put the tubing into a container of water...

Страница 149: ...en working with a system that has the dispenser installed The current state of the instrument server is shown at the top of the page The setup page presents you with a graphical representation of the...

Страница 150: ...is used for filling the syringe and tubing before operation begins Empty is used after operation ends to empty the contents of the syringe and tubing back into the bottle Flush is used for cleaning t...

Страница 151: ...Appendix 3 Dispenser operation 149 Put a reagent bottle into the holder Fit the tubing into the grip Fit the end of the tubing into the reagent bottle Click Fill to fill the tubing with reagent...

Страница 152: ...Appendix 3 Dispenser operation 150 Measurement Close the covers of the dispenser The system should now be ready for operation Proceed with the measurements...

Страница 153: ...e the tubing from the reagent bottle and put it into a container of water and select the Flush command again Note it may be necessary to use a solution of 70 ethanol rather than water for rinsing the...

Страница 154: ...gh quality performance If a needle is bent call the service engineer to get the needle replaced Do not try to re adjust the damaged needle because the quality of dispensing could be affected Both the...

Страница 155: ...permissions Groups and permissions are created during the installation and they cannot be added removed or modified Users can belong to several groups to get more permissions The available groups and...

Страница 156: ...Management is only enabled for users who belong to the Administrators user group With User Management the Wallac 1420 administrator can add remove and edit users and manage the password control Click...

Страница 157: ...ange the password Select the user from the drop down list and click Edit to change the settings of the selected user e g password The Edit user dialogue will appear The functionality is similar to tha...

Страница 158: ...password age cannot be set less than or equal to the Minimum password age If it is not necessary for users to change their password select Password never expires Minimum password age defines the numb...

Страница 159: ...to their current password before they are able to reuse security as their password The minimum is 1 and the maximum is 24 Click Account Lockout to set the lockout properties The Account Lockout dialog...

Страница 160: ...s selected By default the Lockout duration is 60 minutes Audit Trail Note Audit Trail appears only when the enhanced security mode is installed Audit Trail is enabled only for users who belong to the...

Страница 161: ...file The Save command archives the audit trail and deletes the archived entries from the database Only entries that are as old as or older than the selected date are archived Clicking the Export butto...

Страница 162: ...plorer The User can select multiple results from the right pane for archiving Right clicking selected results brings up the Archive menu command the Archive command is found also from the Archive File...

Страница 163: ...e where the user can enter the path of the archive directory The Save command archives results and deletes the archived results from the database Security Settings Note Security Settings appears only...

Страница 164: ...panel However if the administrator has changed the password from the system control panel the Wallac 1420 program will fail to start This is because the Wallac 1420 program has saved the application...

Страница 165: ...163 Index...

Страница 166: ...164...

Страница 167: ...the emission filter slide 95 Column width changing 59 Columns of wells 120 Communication Settings 125 Constant voltage mode 76 Control modes Constant voltage mode 76 For fast kinetics 76 For fluoresce...

Страница 168: ...menu 129 Automatic logout 129 File menu Protocol editor 55 File types for export 64 Fill 147 148 Filter CW Lamp 76 78 83 92 Filter emission 73 76 78 80 83 87 91 Filter excitation LANCE 86 Filter excit...

Страница 169: ...lay 18 Logarithmic scale 20 Luminescence counting time 81 Luminometry 80 M Measurement 44 Measurement Protocol editor 44 Measurement height Plate parameters 48 Measurement mode Plate 44 Single well 44...

Страница 170: ...ate tab 40 48 Plate type 40 49 Plate type Miscellaneous settings 119 Plate type selection Eu Sm norm 100 Plate types for stackers 141 Polarizer aperture 83 Preparing the plate Eu Sm norm 101 Preparing...

Страница 171: ...142 Start Protocol editor 55 Start button 23 24 30 133 Start Wizard 26 30 56 67 122 Startup With enhanced security mode 17 Without enhanced security mode 17 Stop button 32 Strip orientation 120 System...

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