PerkinElmer Spotlight 200, Руководство пользователя

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108 . Spotlight 200 User’s Guide
3. When you are ready to collect a spectrum, select Microscope > Stage Move > To
Coordinate
, and enter the calculated coordinates for the marker.
4. Click to scan the marker.
5. Repeat steps 3 and 4 for each marker.
Setting Up Lines and Maps
The ATR Imaging Accessory is particularly useful for scanning lines and maps on a sample,
because it can do so without raising the crystal between each individual marker. We
recommend that you use the following procedure to set up lines and maps in Spectrum
software:
1. Set the stage origin at the center of the crystal before you add the lines and maps.
2. Add the required lines and maps to the image survey.
Make sure that the areas covered by the lines and maps are sufficent to allow for
movement or distortion of the sample when it comes into contact with the crystal.
3. Multiply the X and Y location coordinates of the line or map by a correction factor.
For germanium, the correction factor is 4. This will ensure that the line or map starts at
the correct position on the sample when viewed through the ATR crystal.
4. Multiply the spacing in each line or map by a correction factor.
For germanium, the correction factor is 4. This will extend the line or map to cover the
equivalent area you originally selected.
5. Add extra points or adjust the aperture sizes as required to ensure that all the area
between the individual points is scanned.
You can now collect all the spectra automatically using the Scan Markers option.