PerkinElmer Spotlight 200, Руководство пользователя

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106 . Spotlight 200 User’s Guide
15. Use the
Stage View Range
menu in the
Microscope
menu to select a suitable viewing
area for your sample.
16. Click on the Stage View toolbar to collect an image survey.
17. Note the coordinates of individual points on the image survey where you want to collect
spectra.
You will need these coordinates later to determine how far to move the stage once the
ATR crystal is in position. Refer to
Correcting the Coordinates of Markers
on page 107.
OR
Add lines and maps to survey areas of interest on your sample.
Refer to the on-screen Help for further information.
You must increase the overall length or area of the lines and maps to allow for the ATR
crystal’s refractive index, and also reduce the space between the points to ensure that
the entire surface between them is analyzed. Refer to
Setting Up Lines and Maps
on
page 108.
18. Adjust the infrared apertures to account for the refractive index of the ATR crystal.
Refer to
Adjusting the Aperture Sizes
on page 107.
19. Swing the crystal arm back over the anvil and clamp it in place on the pillars.
Ensure that the crystal does not touch the sample.
20. Focus again on the registration mark.
21. Raise the stage position by the ATR crystal offset value.
This value is marked in µm on the crystal arm. Raising the stage by this value focuses
the infrared beam at the bottom of the crystal. You can use the joystick (taking care
only to move the stage along the Z axis), or select Microscope > Stage Move > To
Coordinate and change the Z-coordinate only.
22. Click to collect a background spectrum.
Ensure that the background spectrum is free from excessive noise. If necessary,
increase the number of accumulations.
23. Lower the crystal arm gently on to the sample and clamp it in place.
24. Slide the force lever from right to left (Figure 41).
25. Focus again on the registration mark, and select Microscope > Set Stage Origin to
set the origin position of the stage.
26. Raise the stage position by the ATR crystal offset value (step 21).