PerkinElmer Spotlight 200, Руководство пользователя

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Techniques for Collecting Spectra . 105
5. Install the ATR Imaging Accessory on the sample stage, and level it.
Refer to the
ATR Imaging Accessory User’s Guide
(L1050048).
6. Clamp the ATR crystal arm to the two pillars (Figure 41) and check that there is a gap
of a few millimeters between the tip of the crystal and the anvil.
7. Remove the dust cover over the crystal.
8. Select the Reflectance sampling mode on the Setup Microscope Basic tab.
9. Raise the stage, and use the joystick and the Auto-Focus option to focus the microscope
on the line scribed on the crystal arm.
10. Move the stage along this line until the center of the crystal is visible in the Camera
View.
11. Center, and focus on, the registration mark at the center of the crystal’s surface (Figure
42).
You may need to adjust the illumination to see the registration mark clearly.
Figure 42 Image of ATR crystal registration mark
NOTE: From this point onward, do not adjust the stage X and Y positions using the joystick.
12. Select Microscope > Set Stage Origin to set the origin position of the stage.
13. Lift and swing the crystal arm away from the field of view, and place your sample on
the anvil.
We recommend that you fix your sample in position to prevent movement during the
analysis, for example by using adhesive tape.
14. Without moving the X and Y position of the stage, find the area in your sample that you
want to analyze.
It is very important not to use the joystick to move the X and Y position of the sample
stage. Adjust the sample position by moving the sample directly with tweezers, or use
the mini-stage adjusters to center the area of interest (Figure 41).
NOTE:
For best results, position the sample so that the area of interest is close to the center
of the image. Remember that the area you can analyze is limited by the diameter of
the ATR crystal.