NOVA BIOMEDICAL Stat Profile PRIME, Инструкция по эксплуатации

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B-5
Appendix B
Appendix B
B.1.6 Glucose
Glucose measurement is based on the level of H
2
O
2
produced during the enzymatic reaction between glucose
and oxygen molecules in the presence of the glucose oxidase
enzyme. The reaction is described by the following equation:
Glucose Oxidase
G O
2
––--–––––––––––> Gluconic acid + H
2
O
2
Equation 9
At a constant potential of 0.70 volts, electroactive H
2
O
2
is
oxidized at the surface of the platinum anode as follows:
H
2
O
2
––––––––> 2H
+
+ O
2
+ 2e
-
Equation 10
The current generated by the flow of electrons at the
surface of the platinum sensor is proportional to the glucose
concentration of the sample.
B.1.7 Lactate
Lactate measurement is based on the level of H
2
O
2
produced
during the enzymatic reaction between lactate and oxygen
molecules in the presence of the lactate oxidase enzyme.
The reaction is described by the following equation:
Lactate Oxidase
Lactate + O
2
–––––––––––––> Pyruvate acid +
H
2
O
2
Equation 11
At a constant potential of 0.70 volts, electroactive H
2
O
2
is
oxidized at the surface of the platinum anode as follows:
H
2
O
2
––––––––> 2H
+
+ O
2
+ 2e
-
Equation 12
The current generated by the flow of electrons at the
surface of the platinum sensor is proportional to the lactate
concentration of the sample.